Project description:determine the molecular effect of yarrow and marigold supercritical extracts in gene expression of pancreatic cancer cells (MIA PaCa-2)
Project description:determine the molecular effect of yarrow and marigold supercritical extracts in gene expression of pancreatic cancer cells (MIA PaCa-2)
Project description:IGF2BP3-bound transcripts were identified by RNA immunoprecipitation and sequencing (RIP-Seq) and bioinformatics analysis. A pancreatic cancer cell line S2-013 was cultured on fibronectin. We performed RIP of IGF2BP3 using anti-IGF2BP3 antibody from the fibronectin-stimulated S2-013 extracts, and identified the associated mRNAs using a next generation sequencer.
Project description:KHSRP-bound transcripts were identified by RNA immunoprecipitation and sequencing (RIP-Seq) and bioinformatics analysis. A pancreatic cancer cell line S2-013 was cultured on fibronectin. We performed RIP of KHSRP using anti-KHSRP antibody from the fibronectin-stimulated S2-013 extracts, and identified the associated RNAs using a next generation sequencer.
Project description:Members of the Caryophyllaceae Juss. family possess anabolic, adaptogenic, radioprotective, antitumoral and hemorheological properties and have been used in medical practice for the treatment of various inflammatory disorders. Macrophages are key immune cells that coordinate the inflammatory reaction. To identify the effects of Silene sendtneri and Silene roemeri on macrophage activation, primary human macrophages were cultured with extracts of these plants .We used microarrays to determine the global expression pattern induced by the extracts in macrophages.