Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Neutrophil extracellular traps activate IL-8 and IL-1 expression in human bronchial epithelia

ABSTRACT: Neutrophil extracellular traps activate IL-8 and IL-1 expression in human bronchial epithelia

PROVIDER: PRJNA511873 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8370173.fastq.gz	Fastqsanger.gz
	SRR8370174.fastq.gz	Fastqsanger.gz
	SRR8370175.fastq.gz	Fastqsanger.gz
	SRR8370176.fastq.gz	Fastqsanger.gz
	SRR8370177.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Neutrophil extracellular traps activate IL-8 and IL-1 expression in human bronchial epithelia

Project description:Human Broncial Epithilal cells (HBEs) exposed to NETs compared to HBEs exposed to PBS show upregulatation of genes, most of which were related to IL-1 family members and GM-CSF.

2020-04-01 | GSE124378 | GEO

Neutrophil extracellular traps activate IL-8 and IL-1 expression in human bronchial epithelia.

Project description:Neutrophil extracellular traps (NETs) provide host defense but can contribute to the pathobiology of diverse human diseases. We sought to determine the extent and mechanism by which NETs contribute to human airway cell inflammation. Primary normal human bronchial epithelial cells (HBEs) grown at air-liquid interface and wild-type (wt)CFBE41o- cells (expressing wtCFTR) were exposed to cell-free NETs from unrelated healthy volunteers for 18 h in vitro. Cytokines were measured in the apical supernatant by Luminex, and the effect on the HBE transcriptome was assessed by RNA sequencing. NETs consistently stimulated IL-8, TNF-α, and IL-1α secretion by HBEs from multiple donors, with variable effects on other cytokines (IL-6, G-CSF, and GM-CSF). Expression of HBE RNAs encoding IL-1 family cytokines, particularly IL-36 subfamily members, was increased in response to NETs. NET exposure in the presence of anakinra [recombinant human IL-1 receptor antagonist (rhIL-1RA)] dampened NET-induced changes in IL-8 and TNF-α proteins as well as IL-36α RNA. rhIL-36RA limited the increase in expression of proinflammatory cytokine RNAs in HBEs exposed to NETs. NETs selectively upregulate an IL-1 family cytokine response in HBEs, which enhances IL-8 production and is limited by rhIL-1RA. The present findings describe a unique mechanism by which NETs may contribute to inflammation in human lung disease in vivo. NET-driven IL-1 signaling may represent a novel target for modulating inflammation in diseases characterized by a substantial NET burden.

| S-EPMC7468846 | biostudies-literature

Transcription profiling by array of human bronchial epithelial cells stimulated with IL-13

Project description:Human bronchial epithelial cells were stimulated without or with 100 ng/mL IL-13 for 24 hours.

2012-07-05 | E-MEXP-3353 | biostudies-arrayexpress

Exploring intrinsic variability between cultured nasal and bronchial epithelia in cystic fibrosis

Project description:Exploring intrinsic variability between cultured nasal and bronchial epithelia in cystic fibrosis

| PRJNA1010116 | ENA

RNA sequencing of healthy human airway epithelia with IL-13 treatment time course

Project description:The goal of this study was to examine changes to gene expression induced by IL-13 treatment of air-liquid interface cultures of healthy primary human airway epithelia over time.

2023-08-16 | GSE240741 | GEO

IL-13 stimulation of primary human bronchial epithelial cells in control individuals and patients with asthma.

Project description:We performed RNA sequencing of gene expression of differentiated primary human bronchial epithelial cells derived from control and asthmatic patients, stimulated with IL-13. The Type 2 Asthma mediator IL-13 was described to induce airway hyperresponsiveness, goblet cell metaplasia, mucus hypersecretion and airway remoddeling including impairment of epithelial barrier integrity. We investigated differential expression of SARS-CoV-2 related host gene expression as well as genes involved in N-linked glycosylation upon IL-13 in bronchial epithelial cells. Top IL-13 affected pathways included ion- and transmembrane transport, lipid metabolic processed and protein glycosylation.

2023-01-30 | GSE206510 | GEO

Transcriptome profile of NON CF (normal) bronchial epithelial cells treated with IL-4

Project description:To analyse gene expression differences between differentiated bronchial epithelial cells kept under control conditions or treated for 72 hours with IL-4 (10 ng/ml)

2022-10-19 | GSE182955 | GEO

Expression data from human bronchial epithelial cells (treatment with IL-4)

Project description:Goblet cell hyperplasia (GCH) is a feature of respiratory diseases characterized by mucus hypersecretion. GCH is driven by Th2 cytokines such as IL-4. To investigate the gene expression changes associated with GCH, we treated human bronchial epithelial cells with IL-4. By microarray analysis and functional studies, we found that IL-4 promotes a profound change that results in enhanced bicarbonate secretion. This change may be required to support mucus release.

2016-11-09 | GSE78914 | GEO

IL-13 and EGF receptor signaling in normal human bronchial epithelial cells

Project description:To investigate the role of interleukin-13 (IL-13) and the epidermal growth factor (EGF) receptor pathway in controlling mucus metaplasia, normal human bronchial epithelial (NHBE) cells were cultured on air-liquid interface for 14 days and were treated with IL-13, anti-EGFR antibody or both during the final 48 h of culture. Keywords: Stimulus response

2006-11-01 | GSE4804 | GEO

Exploring intrinsic variability between cultured nasal and bronchial epithelia in cystic fibrosis

Project description:The nasal and bronchial epithelium are unified parts of the respiratory tract that are affected in the monogenic disorder cystic fibrosis (CF). Recent studies have uncovered that nasal and bronchial tissues exhibit intrinsic variability, including differences in mucociliary cell composition and expression of unique transcriptional regulatory proteins which relate to germ layer origin. In the present study, we explored transcriptomic differences between cultured nasal and bronchial epithelial cells from people with CF. Comparison of air-liquid interface-differentiated epithelial cells from subjects with CF revealed distinct mucociliary differentiation states of nasal and bronchial cultures. Moreover, using RNA sequencing we identified cell type-specific signature transcription factors in differentiated nasal and bronchial epithelial cells.

2023-11-21 | GSE241801 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data