Project description:OsPAO5 mainly functions to oxidize spermine and produce H2O2,which finally affects mesocotyl elongation in rice. Meanwhile ethylene and polyamines synthesis crosstalk has been reported in competing the common substrate. To further confirm the relationship between H2O2 and ethylene in contributing to the mesocotyl length, we conducted a transcriptome comparison between pao5-1 vs NIP, ACC-treatment vs Mock (NIP) and H2O2-treatment vs Mock (NIP).
Project description:miRNAs were enriched from HEK293T cells using the ambion FLASHPAGE fractionater after mock transfection, Ago2 transfection, and FLAG-Ago2 IP. miRNAs were labeled and hybridized: Ago2 transfected vs. mock transfection and Ago2 transfected vs. Ago2 IPs. Set of arrays that are part of repeated experiments Keywords: Biological Replicate
Project description:Murine MDSCs isolated from the spleens of Lewis lung carcinoma mice were treated with or without WGP, and then miRNA array was used to analyze the differentailly expressed miRNAs. Murine MDSCs were isolated from the spleens of Lewis lung carcinoma tumor-bearing mice, and the sorted MDSCs were stimulated with or without 100 µg/ml WGP for 24 h. Then, the total RNA was extracted to perform miRNA array to analyze the differentially expressed miRNAs in MDSCs treated with or without WGP
Project description:2091 mock vs. 2091 E2F4 IP - Human 13K promoter microarray 2091 cells were serum starved for 72hours and fixed with formaldehyde. Keywords: repeat sample