Project description:With anti-TET1 immunoprecipitated chromatin from mouse frontal cortices, we generated TET1 binding maps with 4496 high-confidence peaks and approximately 90% of TET1 peaks were within intergenic regions.
Project description:In order to assess Tet1 binding, we first generated a Flag tagged Tet1 ES cells and then knocked out Dnmt3a in the [WT, Tet1-Flag] cells. By Tet1 ChIP and Flag ChIP, we showed that Tet1 binding was complementary to Dnmt3a. And Tet1 binding was not affected or slightly increased at majority of its targets.
Project description:Global gene expression profile of Tet1 knocout cortex or hippocampus is compared to wild-type cortex or hippocampus. All mice used are naM-CM-/ve and of mixed 129 C57BL6 backgound. Tet1 KO in brain cortex and hippocampus