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Two distinct ontogenies confer heterogeneity to mouse brain microglia

ABSTRACT: Two distinct ontogenies confer heterogeneity to mouse brain microglia

PROVIDER: PRJNA513193 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8401080.fastq.gz	Fastqsanger.gz
	SRR8401081.fastq.gz	Fastqsanger.gz
	SRR8401082.fastq.gz	Fastqsanger.gz
	SRR8401084.fastq.gz	Fastqsanger.gz
	SRR8401085.fastq.gz	Fastqsanger.gz

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Two distinct ontogenies confer heterogeneity to mouse brain microglia

Project description:Hoxb8 mutant mice show compulsive behavior similar to trichotillomania, a human obsessive-compulsive-spectrum disorder. The only Hoxb8 lineage-labeled cells in the brains of mice are microglia, suggesting that defective Hoxb8 microglia caused the disorder. What is the source of the Hoxb8 microglia? It has been posited that all microglia progenitors arise at embryonic day (E) 7.5 during yolk sac hematopoiesis, and colonize the brain at E9.5. In contrast, we show the presence of two microglia subpopulations: canonical, non-Hoxb8 microglia and Hoxb8 microglia. Unlike non- Hoxb8 microglia, Hoxb8 microglia progenitors appear to be generated during the second wave of yolk sac hematopoiesis, then detected in the aorto-gonad-mesonephros (AGM) and fetal liver, where they are greatly expanded, prior to infiltrating the E12.5 brain. Further, we demonstrate that Hoxb8 hematopoietic progenitor cells taken from fetal liver are competent to give rise to microglia in vivo. Although the two microglial subpopulations are very similar molecularly, and in their response to brain injury and participation in synaptic pruning, they show distinct brain distributions which might contribute to pathological specificity. Non-Hoxb8 microglia significantly outnumber Hoxb8 microglia, but they cannot compensate for the loss of Hoxb8 function in Hoxb8 microglia, suggesting further crucial differences between the two subpopulations.

2019-01-07 | GSE124710 | GEO

Two distinct ontogenies confer heterogeneity to mouse brain microglia.

Project description:Hoxb8 mutant mice show compulsive behavior similar to trichotillomania, a human obsessive-compulsive-spectrum disorder. The only Hoxb8 lineage-labeled cells in the brains of mice are microglia, suggesting that defective Hoxb8 microglia caused the disorder. What is the source of the Hoxb8 microglia? It has been posited that all microglia progenitors arise at embryonic day (E) 7.5 during yolk sac hematopoiesis, and colonize the brain at E9.5. In contrast, we show the presence of two microglia subpopulations: canonical, non-Hoxb8 microglia and Hoxb8 microglia. Unlike non-Hoxb8 microglia, Hoxb8 microglia progenitors appear to be generated during the second wave of yolk sac hematopoiesis, then detected in the aorto-gonad-mesonephros (AGM) and fetal liver, where they are greatly expanded, prior to infiltrating the E12.5 brain. Further, we demonstrate that Hoxb8 hematopoietic progenitor cells taken from fetal liver are competent to give rise to microglia in vivo Although the two microglial subpopulations are very similar molecularly, and in their response to brain injury and participation in synaptic pruning, they show distinct brain distributions which might contribute to pathological specificity. Non-Hoxb8 microglia significantly outnumber Hoxb8 microglia, but they cannot compensate for the loss of Hoxb8 function in Hoxb8 microglia, suggesting further crucial differences between the two subpopulations.

| S-EPMC6053660 | biostudies-literature

Correction: Two distinct ontogenies confer heterogeneity to mouse brain microglia (doi: 10.1242/dev.152306).

Project description: Not available

| S-EPMC6382007 | biostudies-literature

Heterogeneity of Brain Metastasis-associated Microglia and Macrophages

Project description:Heterogeneity of Brain Metastasis-associated Microglia and Macrophages

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Heterogeneity of Brain Metastasis-associated Microglia and Macrophages

Project description:Brain metastasis (BrM) represents a challenging clinical issue. The most common treatment options are surgery and irradiation. Little is known about the complex cellular and molecular microenvironment of BrM, thus lacking molecular targets to combat this dismal disease. It is known that macrophages from the periphery (monocyte-derived macrophages, MDM) and microglia, the brain-resident macrophages, comprise the most abundant stromal cell types in BrM. However, it is not known if both cell types represent a homogeneous cell population with redundant functions or if there are differences due to their ontologic origin. Besides breast cancer and melanoma, the highest incidence of BrM can be found in lung cancer. To gain deeper insight into the myeloid immune landscape and the heterogeneity of BrM-associated immune cells, we here provide single cell RNA Seq data of microglia and MDMs which were associated with a lung cancer (LuCa) BrM, as well as with an irradiated LuCa BrM. Cells were FACS purified into 384 well plates, snap frozen on dry ice and single cell RNA-Seq performed. Together, our data hint towards cell type-specific heterogeneity both, before and upon application of irradiation.

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Self-renewal of Differentiated Microglia Involves Two Distinct Mechanisms

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Self-renewal of Differentiated Microglia Involves Two Distinct Mechanisms II

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Single-cell transcriptomics reveals distinct microglia signatures under inflammation

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2018-09-10 | GSE115571 | GEO

Single-Cell Analysis Uncovers Dramatic Alteration of Microglia Heterogeneity after Ischemic Stroke

Project description:Microglia are resident myeloid cells in the central nervous system (CNS) that regulate homeostasis and protect CNS from damage and infection. The phenotype of microglia is critical in regulating the propagation and resolution of inflammatory responses after ischemic stroke. However, little is known of its complexity and heterogeneity in the above context. Here, using single-cell RNA sequencing (scRNA-seq) of over 11,000 cells from the mice ischemic brain tissues, we demonstrated a previously undefined molecular heterogeneity among microglia clusters following ischemic stroke.

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Self-renewal of Differentiated Microglia Involves Two Distinct Mechanisms

Project description:single cell gene expression profiling of microglia after peripheral nerve injury.

2022-12-18 | GSE142404 | GEO