Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparative Analysis of Antifungal Mechanism of Lauryl betaine in the Human Fungal Pathogens Cryptococcus neoformans and Malassezia restricta

ABSTRACT: Comparative Analysis of Antifungal Mechanism of Lauryl betaine in the Human Fungal Pathogens Cryptococcus neoformans and Malassezia restricta

PROVIDER: PRJNA513301 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Comparative Analysis of Antifungal Mechanism of Lauryl betaine in the Human Fungal Pathogens Cryptococcus neoformans and Malassezia restricta

Project description:Purpose: Understanding the mechanism of action of lauryl betaine against Cryptococcus neoformans and Malassezia restricta. Methods: The transcriptome profile of the lauryl betaine-treated cells compared to that of untreated cells were generated by RNA-seq Illumina Hiseq. Results: A number of genes were differentially expressed in the lauryl betaine-treated cells, which include genes involved in ergosterol biosynthetic pathway.

2019-08-22 | GSE124734 | GEO

Malassezia restricta strain:KCTC 27527

Project description:Malassezia restricta KCTC 27527

| PRJNA270302 | ENA

Malassezia restricta CBS 7877

Project description:Malassezia restricta CBS 7877 genome, complete sequence

| PRJNA474956 | ENA

Understanding the Mechanism of Action of the Anti-Dandruff Agent Zinc Pyrithione against Malassezia restricta.

Project description:Purpose: Understanding the Mechanism of Action of the Anti-Dandruff Agent Zinc Pyrithione against Malassezia restricta. Methods: The transcriptome profile of the ZPT-treated M. restricta cells compared to that of untreated cells were generated by RNA-Seq using Illumina HiSeq. Generated raw reads that passed quality filters were mapped to the reference genome. Mapped reads were counted by featureCounts in Subread package v1.4.3 and the relative transcript abundance was TPM-normalized. Results: A number of genes were differentially expressed in the ZPT-treated cells, which include genes involved in zinc transporter, mitochondirial function, TCA cycle, electron transport chain and lipase.

2018-12-25 | GSE112036 | GEO

Malassezia restricta strain:KCTC 27527

Project description:Malassezia restricta strain:KCTC 27527 Genome sequencing

| PRJNA477735 | ENA

Antifungal Mechanism of Action of Lauryl Betaine Against Skin-Associated Fungus <i>Malassezia restricta</i>.

Project description:Betaine derivatives are considered major ingredients of shampoos and are commonly used as antistatic and viscosity-increasing agents. Several studies have also suggested that betaine derivatives can be used as antimicrobial agents. However, the antifungal activity and mechanism of action of betaine derivatives have not yet been fully understood. In this study, we investigated the antifungal activity of six betaine derivatives against Malassezia restricta, which is the most frequently isolated fungus from the human skin and is implicated in the development of dandruff. We found that, among the six betaine derivatives, lauryl betaine showed the most potent antifungal activity. The mechanism of action of lauryl betaine was studied mainly using another phylogenetically close model fungal organism, Cryptococcus neoformans, because of a lack of available genetic manipulation and functional genomics tools for M. restricta. Our genome-wide reverse genetic screening method using the C. neoformans gene deletion mutant library showed that the mutants with mutations in genes for cell membrane synthesis and integrity, particularly ergosterol synthesis, are highly sensitive to lauryl betaine. Furthermore, transcriptome changes in both C. neoformans and M. restricta cells grown in the presence of lauryl betaine were analyzed and the results indicated that the compound mainly affected cell membrane synthesis, particularly ergosterol synthesis. Overall, our data demonstrated that lauryl betaine influences ergosterol synthesis in C. neoformans and that the compound exerts a similar mechanism of action on M. restricta.

| S-EPMC6691833 | biostudies-literature

Genomic analysis of ketoconazole resistance in the dandruff-associated cutaneous yeast Malassezia restricta.

Project description:To understand ketoconazole resistance mechanism in M. restricta, we investigated differentially expressed genes in the azole resistant isolates using transcriptome profile. Transcriptomes of ketoconazole susceptible and resistant M. restricta cells in the absence and precence of ketoconazole were compared and analyzed by RNA sequencing.

2020-12-05 | GSE141548 | GEO

Identification and characterization of a novel double-strand RNA mycovirus from Malassezia restricta KCTC 27540

Project description:To understand the overall effect of the virus on fungal host, we compared the transcriptomes between virus-infected and virus-cured M. restricta strain by using RNA-Seq.

2020-08-18 | GSE138985 | GEO

Genomic analysis of ketoconazole resistance in the dandruff-associated cutaneous yeast Malassezia restricta.

Project description:Genomic analysis of ketoconazole resistance in the dandruff-associated cutaneous yeast Malassezia restricta.

| PRJNA593855 | ENA

Understanding the Mechanism of Action of the Anti-Dandruff Agent Zinc Pyrithione against Malassezia restricta.

Project description:Understanding the Mechanism of Action of the Anti-Dandruff Agent Zinc Pyrithione against Malassezia restricta.

| PRJNA438989 | ENA