Project description:To investigate whether natural TAG was decoded by stably transfected Mb pyltRNA in transgenic cells, their cell lysate was subjected to ribosome profiling, examining the physical locations of ribosomes. HEK293T cells were taken as control.
Project description:To investigate whether natural TAG can be decoded by stably transfected pyltRNA, whole cell lysate of transgenic cells was collected 48h after the addition of NAEK and subjected to ribosome profiling, examining the physical locations of ribosomes along 15662 TAG terminated transcripts. And HEK293T cells were taken as control.
Project description:We performed ribosome profiling in HEK293T cells with treatment with either C13-benzamidyl cycloheximide (hereafter "benzamide") or cycloheximide.
Project description:SUM1315 were treated with harringtonine, and an untreated control sample was taken. Ribosome profiling was performed on these samples.
Project description:Mouse primary B cells were treated with 10 uM GC7 or vehicle control and processed for ribosome profiling. During harvesting, samples were spiked with S. cerevisiae lysate containing stable polysomes.
Project description:SUM1315 were treated with harringtonine, and an untreated control sample was taken. Ribosome profiling was performed on these samples. 1 treated and 1 untreated sample