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EOMES-dependent epigenetic reprogramming during CD8 T cell development leads to acquisition of memory traits (ChIP-Seq)

ABSTRACT: EOMES-dependent epigenetic reprogramming during CD8 T cell development leads to acquisition of memory traits (ChIP-Seq)

PROVIDER: PRJNA514302 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8428760.fastq.gz	Fastqsanger.gz
	SRR8428770.fastq.gz	Fastqsanger.gz
	SRR8428761.fastq.gz	Fastqsanger.gz
	SRR8428771.fastq.gz	Fastqsanger.gz
	SRR8428762.fastq.gz	Fastqsanger.gz

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EOMES-dependent epigenetic reprogramming during CD8 T cell development leads to acquisition of memory traits

Project description:EOMES-dependent epigenetic reprogramming during CD8 T cell development leads to acquisition of memory traits

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EOMES-dependent epigenetic reprogramming during CD8 T cell development leads to acquisition of memory traits (RNA-Seq)

Project description:Memory CD8 T cells have a unique ability to provide lifelong immunity against pathogens. Although memory features generally arise after a challenge with foreign antigen, naïve CD8 single positive (SP) thymocytes may already acquire phenotypic and functional characteristics of memory cells in response to cytokines such as interleukin-4. This process is associated with the induction of the T-box transcription factor Eomesodermin (EOMES). However, the underlying molecular mechanisms remain ill-defined. Using epigenomic profiling, we show that these “innate memory” CD8SP cells acquire only a portion of the active enhancer repertoire of conventional memory cells. This reprograming was secondary to EOMES recruitment, mostly to RUNX3-bound enhancers. Furthermore, we identified direct interaction between EOMES and BRG1 and showed that in vivo acquisition of EOMES-dependent program by CD8SP thymocytes was dependent on this chromatin remodeling factor. In conclusion, our results support a strong epigenetic basis for EOMES-driven establishment of CD8 T cell unconventional memory program.

2019-06-06 | GSE124913 | GEO

EOMES-dependent epigenetic reprogramming during CD8 T cell development leads to acquisition of memory traits (ChIP-Seq)

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EOMES-dependent epigenetic reprogramming during CD8 T cell development leads to acquisition of memory traits (ATAC-Seq)

2019-06-06 | GSE124911 | GEO

EOMES-dependent epigenetic reprogramming during CD8 T cell development leads to acquisition of memory traits (RNA-Seq)

Project description:EOMES-dependent epigenetic reprogramming during CD8 T cell development leads to acquisition of memory traits (RNA-Seq)

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EOMES-dependent epigenetic reprogramming during CD8 T cell development leads to acquisition of memory traits (ATAC-Seq)

Project description:EOMES-dependent epigenetic reprogramming during CD8 T cell development leads to acquisition of memory traits (ATAC-Seq)

| PRJNA514300 | ENA

EOMES interacts with RUNX3 and BRG1 to promote innate memory cell formation through epigenetic reprogramming

Project description:Memory CD8+ T cells have the ability to provide lifelong immunity against pathogens. Although memory features generally arise after challenge with a foreign antigen, naïve CD8 single positive (SP) thymocytes may acquire phenotypic and functional characteristics of memory cells in response to cytokines such as interleukin-4. This process is associated with the induction of the T-box transcription factor Eomesodermin (EOMES). However, the underlying molecular mechanisms remain ill-defined. Using epigenomic profiling, we show that these innate memory CD8SP cells acquire only a portion of the active enhancer repertoire of conventional memory cells. This reprograming is secondary to EOMES recruitment, mostly to RUNX3-bound enhancers. Furthermore, EOMES is found within chromatin-associated complexes containing BRG1 and promotes the recruitment of this chromatin remodelling factor. Also, the in vivo acquisition of EOMES-dependent program is BRG1-dependent. In conclusion, our results support a strong epigenetic basis for the EOMES-driven establishment of CD8+ T cell innate memory program.

2019-06-06 | PXD014142 | Pride

EOMES-dependent epigenetic reprogramming during CD8 T cell development leads to acquisition of memory traits

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-06-06 | GSE124914 | GEO

Eomes broadens the scope of CD8 T cell memory by inhibiting apoptosis in low-affinity cells

Project description:The memory CD8 T cell pool must select for high-affinity clones to efficiently counter re-infection yet must retain a level of clonal diversity to allow recognition of pathogens with mutated immuno-dominant epitopes. How this is mediated is unclear, especially in the context of a selective drive for antigen-affinity. We find that low-affinity memory exclusively depends on the transcription factor Eomes in the first days after antigen encounter. Eomes is induced at low activating signal strength and directly drives transcription of the pro-survival protein Bcl-2. At higher signal intensity T-bet is induced which suppresses Bcl-2, generating a survival advantage for low-affinity cells. High-affinity cells form memory independent of Eomes and have a proliferative advantage over low-affinity cells, which compensates for their survival deficit. Thus, we demonstrate on a molecular level how sufficient diversity of the memory pool is established in an environment of affinity-based selection.

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Tbet-Eomes

Project description:Tbet-Eomes

| PRJNA706657 | ENA