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Effector differentiation potential determined at the level of human CD8+ naïve T cell subsets

ABSTRACT: Effector differentiation potential determined at the level of human CD8+ naïve T cell subsets

PROVIDER: PRJNA515252 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8443640.fastq.gz	Fastqsanger.gz
	SRR8443650.fastq.gz	Fastqsanger.gz
	SRR8443641.fastq.gz	Fastqsanger.gz
	SRR8443651.fastq.gz	Fastqsanger.gz
	SRR8443642.fastq.gz	Fastqsanger.gz

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Effector differentiation potential determined at the level of human CD8+ naïve T cell subsets [microarray]

Project description:Antigen recognition by CD8+ naïve T cells induces immune activation and rapid effector differentiation that ensures elimination of virally-infected and malignant cells. While signals from the extracellular milieu and downstream of the TCR have long been known to play a crucial role, it is currently unknown whether heterogeneity within the naïve CD8+ T cell pool shapes the potency of effector differentiation. We report the identification of two discrete subsets of human CD8+ TN cells, defined by positive and negative expression of the chemokine receptor CXCR3, with different potential to generate fully-differentiated effector T cells following antigen-specific stimulation. The CXCR3+ are more abundant than the CXCR3– naïve in peripheral blood and lymphoid tissues, retain enhanced expression of effector-related genes ex vivo and display features of the T cell receptor chain repertoire indicating enhanced capacity to bind the peptide:major histocompatibility complex. Likewise, a phenotypically equivalent murine CD8+ naïve population expressing high levels of CD5, a surrogate indicator of enhanced TCR sensitivity for self, preferentially generates short-lived effector cells following infection. Our findings imply that CD8+ T cell effector differentiation potential in humans is shaped by heterogeneity in the preimmune repertoire

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Effector differentiation potential determined at the level of human CD8+ naïve T cell subsets [microarray]

Project description:Effector differentiation potential determined at the level of human CD8+ naïve T cell subsets [microarray]

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Effector differentiation potential determined at the level of human CD8+ naïve T cell subsets [RNA-Seq]

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Effector differentiation potential determined at the level of human CD8+ naïve T cell subsets [RNA-Seq]

Project description:Effector differentiation potential determined at the level of human CD8+ naïve T cell subsets [RNA-Seq]

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Effector differentiation potential determined at the level of human CD8+ naïve T cell subsets

Project description:This SuperSeries is composed of the SubSeries listed below.

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Expression data for effector T cells

Project description:In this study, we examined differential gene expression in naïve human CD4+ T cells, as well as in effector Th1, Th17-negative and Th17-enriched CD4- T cell subsets. We observed a marked enrichment for increased gene expression in effector CD4+ T cells compared to naive CD4+ among immune-mediated disease oci genes. Within effector T cells, expression of disease-associated genes was increased in Th17-enriched compared to Th17-negative cells. We used microarray to examine the gene expresssion profile and level of human naïve, Th1 and effector T cell subsets. Human PBMCs were isolated and sorted to naïve, CD161-CCR6- and CD161+CCR6+ memory T cells. Naïve T cells were differentiatied to Th1 cells, and CD161-CCR6- and CD161+CCR6+ memory T cells were in vitro expanded for Th17-negative and Th17-enriched effector T cells. The gene profile was compared among naive, Th1, Th17-negative, and Th17-enriched cell subsets.

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Expression data for effector T cells

2012-05-13 | GSE32901 | GEO