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PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells (RNA-seq)

ABSTRACT: PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells (RNA-seq)

PROVIDER: PRJNA515466 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8447830.fastq.gz	Fastqsanger.gz
	SRR8447840.fastq.gz	Fastqsanger.gz
	SRR8447831.fastq.gz	Fastqsanger.gz
	SRR8447841.fastq.gz	Fastqsanger.gz
	SRR8447832.fastq.gz	Fastqsanger.gz

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PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells (ChIP-seq)

Project description:The PLZF transcription factor is essential for osteogenic differentiation of hMSCs, however, its regulation and molecular function during this process is not fully understood. Here we revealed that the ZBTB16 locus encoding PLZF, is repressed by Polycomb (PcG) and H3K27me3 in naïve hMSCs. At the pre-osteoblast stage of differentiation, the locus lost PcG binding and H3K27me3, gained JMJD3 recruitment, and H3K27ac resulting in high expression of PLZF. Subsequently, PLZF was recruited to osteogenic enhancers, influencing H3K27 acetylation and expression of nearby genes important for osteogenic function. Furthermore, we identified a latent enhancer within the ZBTB16/PLZF locus itself that became active, gained PLZF, p300 and Mediator binding and looped to the promoter of the nicotinamide N-methyltransferase (NNMT) gene. The increased expression of NNMT correlated with a decline in SAM levels, which is dependent on PLZF and is required for osteogenic differentiation.

2019-01-17 | GSE125166 | GEO

PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells (RNA-seq)

2019-01-17 | GSE125167 | GEO

PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells (4C-seq)

2019-01-17 | GSE125165 | GEO

PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells

Project description:PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells

| PRJNA515440 | ENA

PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells (ChIP-seq)

Project description:PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells (ChIP-seq)

| PRJNA515463 | ENA

PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells (4C-seq)

Project description:PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells (4C-seq)

| PRJNA515464 | ENA

Proteomics analysis of physiological effect of ZBTB16 (PLZF) on osteogenic differentiation of human valve interstitial cells (VICs) by DDA PASEF on TimsToF Pro

Project description:During fundamental proteotranscriptomic analysis of human valve interstitial cells (VICs) osteogenic differentiation, we revealed ZBTB16 (PLZF) as one of the most upregulated proteins. Thus, to investigate its physiological role in VICs osteogenic differentiation we performed its overexpression by lentiviral transduction with subsequent proteomics analysis. Despite obvious PLZF overexpression and its reinforcing effect on osteogenic differentiation, we revealed surprisingly small amount of differentially expressed proteins that assumed to be the targets of PLZF.

2022-10-21 | PXD031572 | Pride

PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells.

Project description:The PLZF transcription factor is essential for osteogenic differentiation of hMSCs; however, its regulation and molecular function during this process is not fully understood. Here, we revealed that the ZBTB16 locus encoding PLZF, is repressed by Polycomb (PcG) and H3K27me3 in naive hMSCs. At the pre-osteoblast stage of differentiation, the locus lost PcG binding and H3K27me3, gained JMJD3 recruitment, and H3K27ac resulting in high expression of PLZF. Subsequently, PLZF was recruited to osteogenic enhancers, influencing H3K27 acetylation and expression of nearby genes important for osteogenic function. Furthermore, we identified a latent enhancer within the ZBTB16/PLZF locus itself that became active, gained PLZF, p300 and Mediator binding and looped to the promoter of the nicotinamide N-methyltransferase (NNMT) gene. The increased expression of NNMT correlated with a decline in SAM levels, which is dependent on PLZF and is required for osteogenic differentiation.

| S-EPMC6344081 | biostudies-literature

PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-01-17 | GSE125168 | GEO

PLZF limits enhancer activity during hematopoietic progenitor aging

Project description:PLZF (promyelocytic leukemia zinc finger) is a transcription factor acting as a global regulator of hematopoietic commitment. PLZF displays an epigenetic specificity by recruiting chromatin-modifying factors but little is known about its role in remodeling chromatin of cells committed towards a given specific hematopoietic lineage. In murine myeloid progenitors, we decipher a new role for PLZF in restraining active genes and enhancers by targeting acetylated lysine 27 of Histone H3 (H3K27ac). Functional analyses reveal that active enhancers bound by PLZF are involved in biological processes related to metabolism and associated with hematopoietic aging. Comparing the epigenome of young and old myeloid progenitors, we reveal that H3K27ac variation at active enhancers is a hallmark of hematopoietic aging. Taken together, these data suggest that PLZF, associated with active enhancers, appears to restrain their activity as an epigenetic gatekeeper of hematopoietic aging.

2018-12-21 | GSE124190 | GEO

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