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ABSTRACT: Association Mapping of fertility restorer gene for CMS PET1 in sunflower

PROVIDER: PRJNA515598 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8450170_1.fastq.gz	Fastqsanger.gz
	SRR8450170_2.fastq.gz	Fastqsanger.gz
	SRR8450167_1.fastq.gz	Fastqsanger.gz
	SRR8450167_2.fastq.gz	Fastqsanger.gz
	SRR8450168_1.fastq.gz	Fastqsanger.gz

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The radish Ogura fertility restorer impedes translation elongation along its cognate CMS-causing mRNA

Project description:The control of mRNA translation has been increasingly recognized as a key regulatory step for gene control but clear examples in eukaryotes are still scarce. Nucleo-cytoplasmic male sterilities (CMS) represent ideal genetic models to dissect genetic interactions between the mitochondria and the nucleus in plants. This trait is determined by specific mitochondrial genes and is associated with a pollen sterility phenotype that can be suppressed by nuclear genes known as restorer-of-fertility (Rf) genes. In the study, we focused on the Ogura CMS system in rapeseed and showed that the suppression to male sterility by the PPR-B fertility restorer (also called Rfo) occurs through a specific inhibition of the translation of the mitochondria-encoded cms-causing mRNA orf138. We also demonstrate that PPR-B binds within the coding sequence of orf138 and acts as a ribosome blocker to specifically impede translation elongation along the orf138 mRNA. Rfo is the first recognized fertility restorer shown to act this way. These observations will certainly facilitate the development of synthetic fertility restorers for CMS systems in which efficient natural Rfs are lacking.

2021-10-01 | GSE178543 | GEO

The radish Ogura fertility restorer impedes translation elongation along its cognate CMS-causing mRNA [RNA-seq]

2021-10-01 | GSE178722 | GEO

Transcriptome Sequencing and De Novo Analysis of a Cytoplasmic Male Sterile Line and Its Near-Isogenic Restorer Line in Chili Pepper (Capsicum annuum L.)

Project description:Background: The use of cytoplasmic male sterility (CMS) in F1 hybrid seed production of chili pepper is increasingly popular. However, the molecular mechanisms of cytoplasmic male sterility and fertility restoration remain poorly understood due to limited transcriptomic and genomic data. Therefore, we analyzed the difference between a CMS line 121A and its near-isogenic restorer line 121C in transcriptome level using next generation sequencing technology (NGS), aiming to find out critical genes and pathways associated with the male sterility. Results: We generated approximately 53 million sequencing reads and assembled de novo, yielding 85,144 high quality unigenes with an average length of 643 bp. Among these unigenes, 27,191 were identified as putative homologs of annotated sequences in the public protein databases, 4,326 and 7,061 unigenes were found to be highly abundant in lines 121A and 121C, respectively. Many of the differentially expressed unigenes represent a set of potential candidate genes associated with the formation or abortion of pollen. Conclusions: Our study profiled anther transcriptomes of a chili pepper CMS line and its restorer line. The results shed the lights on the occurrence and recovery of the disturbances in nuclear-mitochondrial interaction and provide clues for further investigations. Anther transcriptomes of a chili pepper CMS line 121A and its nearisogenic restorer line 121C were generated by deep sequencing, using Illumina HiSeq 2000.

2013-07-03 | E-GEOD-45431 | biostudies-arrayexpress

Transcriptome Sequencing and De Novo Analysis of a Cytoplasmic Male Sterile Line and Its Near-Isogenic Restorer Line in Chili Pepper (Capsicum annuum L.)

2013-07-03 | GSE45431 | GEO

Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq.

Project description:Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana) and two restorers ones (Patres and Primépi) were used to identify effective Rf (fertility restorer) genes by next generation sequencing on whole transcriptomes (RNA-seq).

2021-06-30 | E-MTAB-10131 | biostudies-arrayexpress

Brassica napus isolate:CLR6430

Project description:Ogu CMS restorer CLR6430 resequencing

| PRJNA488570 | ENA

The radish Ogura fertility restorer impedes translation elongation along its cognate CMS-causing mRNA

Project description:The radish Ogura fertility restorer impedes translation elongation along its cognate CMS-causing mRNA

| PRJNA739583 | ENA

The radish Ogura fertility restorer impedes translation elongation along its cognate CMS-causing mRNA [RNA-seq]

Project description:The radish Ogura fertility restorer impedes translation elongation along its cognate CMS-causing mRNA [RNA-seq]

| PRJNA740195 | ENA

Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by next generation sequencing of products of Representational Difference Analysis (RDA-seq).

Project description:Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana) and two restorers ones (Patres and Primépi) were used to Representational Difference Analysis (cDNA-RDA) to identify effective Rf (fertility restorer) genes. Anthers at the microspore stage were used as testers while anthers excised at the stage of pollen tetrads were selected as driver. The tester:driver ratio in three subsequent rounds of subtractive hybridization increased from 1:50, and 1:400 to 1:200000. Differential products obtained in the third subtraction of the size range from 200 to 800 bp were sequenced.

2021-06-20 | E-MTAB-10132 | biostudies-arrayexpress

rfppr-Comparing nuclear gene expression in two CMS-systems using microarray technology

Project description:ra05-11_cmsrapeseed - rfppr - -To understand how/if fertility and the mitochondrial background are interlinked. -To understand how the mitochondrial background influences the nuclear gene expression. -Compare and describe the total nuclear gene expression of CMS vs. fertile and CMS vs. Restored. -Describe and analyse genes that differ in expression (of special interest are genes that differs in both comparisons). -Group genes e.g. floral genes, highly expressed genes, transcription factors, nuclear encoded genes targeted for the mitochondrion. -Comparing two CMS-systems to elucidate differences and similarities between them. - Flower buds from three B. napus lines (Pactol, CMS, Rfo PPR) at one developmental stage (stage 8). Keywords: wt vs mutant comparison 6 dye-swap - CATMA arrays

2008-07-31 | E-GEOD-8820 | biostudies-arrayexpress

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