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Determining the impact of the Bromodomain and extra-terminal inhibitor JQ1 on the GATA2 cistrome in castrate-resistant prostate cancer

ABSTRACT: Determining the impact of the Bromodomain and extra-terminal inhibitor JQ1 on the GATA2 cistrome in castrate-resistant prostate cancer

PROVIDER: PRJNA515622 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8450680.fastq.gz	Fastqsanger.gz
	SRR8450681.fastq.gz	Fastqsanger.gz
	SRR8450682.fastq.gz	Fastqsanger.gz
	SRR8450683.fastq.gz	Fastqsanger.gz
	SRR8450676.fastq.gz	Fastqsanger.gz

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Determining the impact of the Bromodomain and extra-terminal inhibitor JQ1 on the GATA2 cistrome in castrate-resistant prostate cancer

Project description:To determine the impact of the Bromodomain and extra-terminal (BET) inhibitor JQ1 on the GATA2 cistrome in castrate-resistant prostate cancer we performed GATA2 ChIP-seq in the presence and absence of JQ1 and assessed differential binding upon JQ1 treatment for 24 hours. We first determined the GATA2 cistrome using our GATA2 ChIP-seq data. Overlapping this with previously published ChIP-seq data for the BET family of proteins (Asangani et al., 2014), we identified a substantial proportion of GATA2 genomic binding sites which are co-occupied by a BET protein. We then quantified differential binding of GATA2 upon JQ1 treatment genome-wide.

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