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RNA-seq and miRNA-seq of testicular peritubular myoid cells, other testicular cells, and other tissue smooth muscle-like cells

ABSTRACT: RNA-seq and miRNA-seq of testicular peritubular myoid cells, other testicular cells, and other tissue smooth muscle-like cells

PROVIDER: PRJNA515817 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8454040.fastq.gz	Fastqsanger.gz
	SRR8454050.fastq.gz	Fastqsanger.gz
	SRR8454060.fastq.gz	Fastqsanger.gz
	SRR8454070.fastq.gz	Fastqsanger.gz
	SRR8454080.fastq.gz	Fastqsanger.gz

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RNA-Seq of testicular peritubular myoid cells, other testicular cells, and other tissue smooth muscle-like cells

Project description:In addition to germ cell population, testes contain several types of somatic cells, including Sertoli cells, Leydig cells, and peritubular myoid (PTM) cells. PTM cells are a type of smooth muscle-like cells and have contraction ability. To identify a marker of PTM cells, we isolated primary cells and subsequently performed RNA-Seq of testicular peritubular myoid cells (PTM_1, PTM_2, PTM_3), testicular Sertoli cells (Sertoli_1, Sertoli_2, Sertoli_3), testicular Leydig cells (Leydig_1, Leydig_2, Leydig_3), testicular germ cells (Germ_1, Germ_2, Germ_3), vascular smooth muscle cells (VSMC_1, VSMC_2, VSMC_3), intestinal smooth muscle cells (ISMC_1, ISMC_2, ISMC_3), and tracheal smooth muscle cells (TSMC_1, TSMC_2, TSMC_3) using BGISEQ-500 platform (BGI, China).

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miRNA-Seq of testicular peritubular myoid cells, other testicular cells, and other tissue smooth muscle-like cells

Project description:In addition to germ cell population, testes contain several types of somatic cells, including Sertoli cells, Leydig cells, and peritubular myoid (PTM) cells. PTM cells are a type of smooth muscle-like cells and have contraction ability. To identify a marker of PTM cells, we isolated primary cells and subsequently performed miRNA-Seq of testicular peritubular myoid cells (PTM_1, PTM_2, PTM_3), testicular Sertoli cells (Sertoli_1, Sertoli_2, Sertoli_3), testicular Leydig cells (Leydig_1, Leydig_2, Leydig_3), testicular germ cells (Germ_1, Germ_2, Germ_3), vascular smooth muscle cells (VSMC_1, VSMC_2, VSMC_3), intestinal smooth muscle cells (ISMC_1, ISMC_2, ISMC_3), and tracheal smooth muscle cells (TSMC_1, TSMC_2, TSMC_3) using BGISEQ-500 platform (BGI, China).

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Effect of heat stress on mouse testicular peritubular myoid cells [miRNA-seq]

Project description:We performed miRNA-Seq of untreated testicular peritubular myoid cells (NC_1, NC_2, NC_3), and peritubular myoid cells that were collected 1 hour (H1_1, H1_2, H1_3) and 6 hours (H6_1, H6_2, H6_3) after heat treatment using BGISEQ-500 platform (BGI, China).

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Project description:We performed RNA-Seq of untreated testicular peritubular myoid cells (NC_1, NC_2, NC_3), and peritubular myoid cells that were collected 1 hour (H1_1, H1_2, H1_3) and 6 hours (H6_1, H6_2, H6_3) after heat treatment using BGISEQ-500 platform (BGI, China).

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Effect of endothelin-1 (ET-1) on mouse testicular peritubular myoid cells [miRNA-Seq]

Project description:PTM cells can be induced to contract by endothelin 1 (ET1). We performed miRNA-Seq of untreated testicular peritubular myoid cells (PTM_1, PTM_2, PTM_3) and ET1-treated peritubular myoid cells (ET1_1, ET1_2, ET1_3) using BGISEQ-500 platform (BGI, China).

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Effect of endothelin-1 (ET-1) on mouse testicular peritubular myoid cells [RNA-Seq]

Project description:PTM cells can be induced to contract by endothelin 1 (ET1). We performed RNA-Seq of untreated testicular peritubular myoid cells (PTM_1, PTM_2, PTM_3) and ET1-treated peritubular myoid cells (ET1_1, ET1_2, ET1_3) using BGISEQ-500 platform (BGI, China).

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