Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The gene expression analysis of TDP knocked down MIN6 cells.

ABSTRACT: The gene expression analysis of TDP knocked down MIN6 cells.

PROVIDER: PRJNA516346 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

The gene expression analysis of TDP knocked down MIN6 cells.

Project description:TAR DNA-binding protein 43 kDa (TDP-43), encoded by TARDBP, is an RNA-binding protein, the nuclear depletion of which is the histopathological hallmark of amyotrophic lateral sclerosis (ALS). Here, we show that ALS subjects have reduced early-phase insulin secretion and that the nuclear localization of TDP-43 is lost in the islets of autopsied ALS pancreas. Loss of TDP-43 inhibits exocytosis, thereby reducing early-phase insulin secretion in a cultured β cell line (MIN6). Using microarray analysis, we identified the genes causing insulin impaired in TDP knocked down MIN6 cells.

2019-01-23 | GSE125424 | GEO

Gene expression data from Min6 cells grown in serum containing and serum free condtions following Hes3 knock down

Project description:The basic helix-loop-helix (bHLH) transcription factor hairy and enhancer of split (Hes3) is a member of the Hes/Hey gene family that regulates developmental processes in progenitor cells from various tissues. We demonstrated the Hes3 expression in mouse pancreatic tissue, suggesting it may have a role in modulating beta-cell function. We employed a transfection approach to address specific functions of Hes3. Hes3 RNA interference opposed the growth of the mouse insulinoma cell line Min6. Western blotting and PCR approaches specifically showed that Hes3 RNA interference opposes the expression of Pdx1 and insulin. Likewise, Hes3 knock down reduced evoked insulin release from Min6 cells. We used microarray analysis to examine differences in gene expression when Hes3 expression was knocked down in cells grown under different culture condtions.

2014-12-23 | E-GEOD-64449 | biostudies-arrayexpress

Gene expression by TRPS1 knocked down in T47D

Project description:Gene expression by TRPS1 knocked down in T47D

| PRJNA408130 | ENA

Epigenome analysis of ALKBH5 knocked down HCT116 cell

Project description:Epigenome analysis of ALKBH5 knocked down HCT116 cell

| PRJNA786900 | ENA

RNA-seq analysis about MuERVL knocked down samples

Project description:RNA-seq analysis about MuERVL knocked down samples

| PRJNA449389 | ENA

Gene expression by BTF3 knocked down in Vcap cells

Project description:Gene expression by BTF3 knocked down in Vcap cells