Project description:Pyrenophora tritici-repentis isolate PtrDW5 extracellular
Proteome mapping of fungus Pyrenophora tritici-repentis (Ptr) extracellular proteins
Sample analysis (in brief):
- extracellular proteins have been separated into 24 fractions by OFFGEL electrophoresis (IEF)
- proteins of each fraction have then been alkylated (iodoacetamide) and trypsin-digested
- peptides of each fraction have then been separated on a C18 column in an LC run and analysed by high-resolution mass spectrometry (LTQ Orbitrap Velos or XL)
