Project description:Sebaceous gland carcinoma is the frequent malignant tumor of the eyelid. However, there is limited understanding of how altered gene expression of sebaceous gland carcinoma of the eyelid related to the pathogenesis. In this study, to identify genes involved in sebaceous gland carcinoma of the eyelid, global-scale gene expression analysis was carried out using a GeneChip® system.
Project description:To identify the molecular background of eyelid sebaceous gland carcinomas (SCs), the integrated whole-exome sequencing and transcriptome sequencing for five eyelid SCs were conducted in this study.
Project description:To screen the related miRNAs in the occurence and development of sebaceous gland carcinoma(SGC) of the eyelid , we have employed Human Agilient microarray expression profiling as a discovery platform to identify differentially expressed miRNAs for SGC and TP53 mtation related miRNAs. Human formalin-fixed paraffin-embedded (FFPE) SGC tissues and para-carcinoma sebaceous gland(SG) FFPE samples from SGC patients were screened using microarray assay. Human FFPE SGC tissues containing TP53 mutation and human FFPE SGC tissues without TP53 mutation were screened using microarray. The overlap of two sets of microarray differentially upregulated miRNAs were selected as our research object.
Project description:Basal cell carcinoma (BCC) is the most frequent malignant tumor of the eyelid. However, there is limited understanding of how altered gene expression of BCC of the eyelid related to the pathogenesis. In this study, to identify genes involved in basal cell carcinoma of the eyelid, global-scale gene expression analysis was carried out using a GeneChip® system.
Project description:Meibomian glands (MGs) are specialized holocrine sebaceous glands embedded in the tarsal plate of the eyelids. The cell heterogeneity of MGs is not well characterized. In this study, we performed a spatial transcriptomic analysis of murine eyelid using the 10X Visium platform.
Project description:We isolated murine sebaceous gland (SG) cells and performed highly targeted single cell RNA-sequencing (scRNA-seq) to identify novel cell subpopulations and to infer lineage relationships.