Single-cell RNA sequencing reveals regulatory mechanism for trophoblast cell-fate divergence in human peri-implantation embryo
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ABSTRACT:
PROVIDER: PRJNA516921 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR8483420_1.fastq.gz | Fastqsanger.gz | |||
| SRR8483420_2.fastq.gz | Fastqsanger.gz | |||
| SRR8483430_1.fastq.gz | Fastqsanger.gz | |||
| SRR8483430_2.fastq.gz | Fastqsanger.gz | |||
| SRR8483440_1.fastq.gz | Fastqsanger.gz |
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Project description:We studied the transcriptomic dynamics underlying human per-implantation development of trophoblast cells.
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Project description:Single cell RNA sequencing of cells from cultured human blastocysts has enabled us to define the transcriptomic landscape of placental trophoblast (TB) that surrounds the epiblast and associated embryonic tissues during the enigmatic day (D) 8 to D12 peri-implantation period before the villous placenta forms. We analyzed the transcriptomes of three early placental cell types, cytoTB (CTB), syncytioTB (STB) and migratoryTB (MTB) picked manually from cultured embryos dissociated with trypsin and were able to follow sub-lineages that emerged from proliferating CTB at the periphery of the conceptus. A unique form of CTB with some features of STB was detectable at D8, while mature STB was at its zenith at D10. A form of MTB with a mixed MTB/CTB phenotype arose around D10. By D12, STB generation was in decline, CTB had entered a new phase of proliferation, and mature MTB cells had begun to move from the main body of the conceptus. Notably, the MTB transcriptome at D12 indicated enrichment of transcripts associated with interferon signaling, migration and invasion, and upregulation of HLA-C, HLA-E, and HLA-G. The STB, which is distinct from the STB of later villous STB, had a phenotype consistent with intense protein export and placental hormone production, as well as migration and invasion. The studies show that TB associated with human embryos is in rapid developmental flux during peri-implantation period when it must invade, signal robustly to the mother to ensure that the pregnancy continues, and make first contact with the maternal immune system.
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