Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression changes in cord blood-derived ECFCs in response to in vitro senescence stimuli for the identification of novel reference genes

ABSTRACT: Gene expression changes in cord blood-derived ECFCs in response to in vitro senescence stimuli for the identification of novel reference genes

PROVIDER: PRJNA517513 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Gene expression changes in cord blood-derived ECFCs in response to in vitro senescence stimuli

Project description:Gene expression changes in cord blood-derived ECFCs in response to in vitro senescence stimuli

| PRJNA672167 | ENA

Gene expression changes in cord blood-derived ECFCs in response to in vitro senescence stimuli for the identification of novel reference genes

Project description:To identify the most suitable reference genes for ECFC senescence studies, we analysed a microarray dataset, which compared the gene expression between proliferating and senescent ECFCs. In addition to replicative senescence, the data included X-ray-induced and Etoposide-induced senescence. We used the geNorm algorithm to identify the most stable genes across all studied conditions.

2019-03-27 | GSE125792 | GEO

Human ECFCs: Cord Blood vs Peripehral Blood ECFCs

Project description:miRNA profiling was carried out using the miRCURY LNA™ microRNA Array (5th gen - hsa, mmu & rno). microRNA profiling of CB and PB-derived ECFCs from 3 independent donors from each cell source. To identify and understand the regulation of endothelial cell functions through comparing miRNA expression profiling of cord blood (CB) derived endothelial colony forming cells (ECFCs) and peripheral blood (PB) ECFCs

2017-03-20 | GSE81574 | GEO

Gene expression changes in cord blood-derived ECFCs in response to in vitro senescence stimuli

Project description:Senescent endothelial cells accumulate in blood vessels during aging and produce the senescence-associated secretory phenotype (SASP). Age-related cardiovascular disease is promoted by SASP chronic inflammation. SASP establishment has been attributed to DNA damage and cGAS activation through cytoplasmic chromatin fragments. Therefore, DNA sensing has been extensively studied in cellular senescence; RNA sensing, on the other hand, remains unexplored. Here, we uncover a pivotal role for RNA accumulation and sensing in endothelial senescence. Our study suggests that intracellular RNA accumulation is a hallmark of senescent endothelial cells. This is associated with activation of RIG-I RNA sensing, and IRF7-driven IFN innate immune response. Moreover, our results revealed that inhibition of IRF7 or RIG-I were sufficient to extend the lifespan and functionality of endothelial cells. These data link the IFN gene signature with RNA accumulation/sensing in senescent endothelium and suggest IRF7 and RIG-I as potential therapeutic targets to delay vascular aging.

2023-10-23 | GSE160166 | GEO

Human umbilical cord blood-derived endothelial colony forming cells under serum-free conditions

Project description:Derivation and expansion of human umbilical cord blood-derived endothelial colony forming cells under serum-free conditions - a transcriptome analysis. Endothelial colony forming cells (ECFCs) were isolated from term umbilical cord blood units. ECFCs were expanded under standard, fetal bovine serum (FBS) containing endothelial medium, or transferred to chemically defined endothelial media without FBS. Microarray expression profiling was applied to compare the transcriptome profiles in FBS-containing versus FBS-free culture. Comparison of the expression patterns of ECFCs that were either cultured in FBS-containing medium or in serum-free medium (five replicates each).

2010-07-29 | E-GEOD-20504 | biostudies-arrayexpress

Human umbilical cord blood-derived endothelial colony forming cells under serum-free conditions

2010-06-29 | GSE20504 | GEO

Analysis of cord blood B cells

Project description:Purified CD19+ B cells isolated from the cord blood were purified in subsets and activated by diferent stimuli and compared by whole genome microarray analysis.

2017-03-01 | GSE78847 | GEO

Analysis of the transcriptome of CD34+38- cord blood cells treated with 3-deazaadenosine (3DA)

Project description:Cord blood cells were cultured in the presence of 10 mM 3-deazaadenosine (3DA) or DMSO. After 9 days, the CD34+38- population was sorted using FACS and subjected to RNAseq. Our previous experiments have shown that treating other cell types with 3DA partially prevents senescence. By performing RNAseq, we show how treating cord blood cells with 3DA affects senescence, stemness and differentiation

2022-07-19 | GSE175770 | GEO

DNA methylation profiling of cord blood progenitor endothelial cells from overweight and GDM pregnancies

Project description:Fetal progenitor endothelial cells (endothelial colony forming cells; ECFC) are recruited for repair, vascular growth and angiogenesis and their high abundance perinatally suggests a function in postnatal vasculogenesis and angiogenesis. In this study we profiled ECFCs from pregnancies of control, overweight and diabetic mothers to study if adverse pregnancies are associated with epigenetic variation in ECFCs.

2021-12-14 | GSE180355 | GEO

Homo sapiens

Project description:Human ECFCs: Cord Blood vs Peripehral Blood ECFCs

| PRJNA322029 | ENA