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Analysis of seeds from Aethionema arabicum Turkish (TUR) and Cyprus (CYP) accession in response to light

ABSTRACT: Analysis of seeds from Aethionema arabicum Turkish (TUR) and Cyprus (CYP) accession in response to light

PROVIDER: PRJNA517709 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8503240.fastq.gz	Fastqsanger.gz
	SRR8503241.fastq.gz	Fastqsanger.gz
	SRR8503232.fastq.gz	Fastqsanger.gz
	SRR8503242.fastq.gz	Fastqsanger.gz
	SRR8503233.fastq.gz	Fastqsanger.gz

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Analysis of seeds from Aethionema arabicum Turkish (TUR) and Cyprus (CYP) accession in response to light

Project description:The seed germination in response to light shows natural variation in two accessions of Aethionema arabicum (Brassicaceae). One accession from Turkey (TUR) germinates well in darkness and light, while another closely related accession from Cyprus (CYP) germinates only in the dark. To understand the transcriptional differences between the two accession, we performed RNA-seq analysis from imbibed seeds, which were kept either in darkness or under light for 23 hours. Results provide the first transcriptional comparison from light-neutral (TUR) and light-sensivite (CYP) seeds kept in darkness or exposed to light.

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Analysis of light induced secondary seed dormancy in Aethionema arabicum, Cyprus accession

Project description:Light inhibits the seed germination in the Cypriot accession (CYP) of Aethionema arabicum. Extended light illumination also induces a secondary seed dormancy that inhibits the germination even if the seeds were transferred back to darkness. This analysis aims to compare the seed transcriptome before or after the dormancy was established by light illumination (100 µmol/m2s; white light). One day light treatment (1dL) inhibits the germination but the seeds are not yet dormant. Seven day (7dL) or 14-day (14dL) long light treatment induces dormancy, and the seeds stay dormant after 7 dal-light plus 7 day-dark (7dL7dD) treatment. To understand the dormancy establishment, transcriptome was compared between non-dormant (1dL) and dormant (7dL, 14dL, 7dL7dD) seed samples.

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Analysis of light induced secondary seed dormancy in Aethionema arabicum, wild type and rgl2 mutant in Cyprus accession

Project description:Light inhibits the seed germination in the Cypriot accession (CYP) of Aethionema arabicum. Extended light illumination also induces a secondary seed dormancy that inhibits the germination even if the seeds were transferred back to darkness. A forward genetic screen identified rgl2 mutant, that show light inhibited germination, but fails to re-enter the secondary dormancy. This analysis aims to compare the seed transcriptome before or after the dormancy was established by light illumination (100 µmol/m2s; white light). One day light treatment (1dL) inhibits the germination but the seeds are not yet dormant. Seven day (7dL) long light treatment induces dormancy in the WT but not in the rgl2 mutant.

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