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Mice gut Metagenome (Arsenic)

ABSTRACT: Mice gut Metagenome (Arsenic)

PROVIDER: PRJNA522840 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11092428_1.fastq.gz	Fastqsanger.gz
	SRR11092428_2.fastq.gz	Fastqsanger.gz
	SRR8613930_1.fastq.gz	Fastqsanger.gz
	SRR8613930_2.fastq.gz	Fastqsanger.gz
	SRR8613929_1.fastq.gz	Fastqsanger.gz

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Project description:Quantitative metaproteomics is a relatively new research field by applying proteomics technique to study microbial proteins of microbiome, and holds the great potential to truly quantify the functional proteins actually expressed by microbes in the biological environment such as gastrointestinal tract. The significant association between arsenic exposure and gut microbiome perturbations has been reported; however, metaproteomics has not yet been applied to study arsenic induced proteome changes of microbiome. Most importantly, to our knowledge, isobaric-labeling based large-scale metaproteomics has not been reported using the advanced database search approaches such as MetaPro-IQ and matched metagenome database search strategies to provide high quantification accuracy and less missing quantification values. In the present study, a new experimental workflow coupled with isobaric labeling and MetaPro-IQ was demonstrated for metaproteomics study of arsenic induced gut microbiome perturbations. The advantages of this workflow were also discussed. For all 18 fecal samples analyzed, 7,611 protein groups were quantified without any missing values. The consistent results of expression profiles were observed between 16S rRNA gene sequencing and metaproteomics. This isobaric labeling based workflow demonstrated the significant improvement of quantitative metaproteomics for gut microbiome study.

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