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Transcriptome Analysis of the different expressed genes affected by ClpP in B. abortus 2308

ABSTRACT: Transcriptome Analysis of the different expressed genes affected by ClpP in B. abortus 2308

PROVIDER: PRJNA523718 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8607560_1.fastq.gz	Fastqsanger.gz
	SRR8607560_2.fastq.gz	Fastqsanger.gz
	SRR8607561_1.fastq.gz	Fastqsanger.gz
	SRR8607561_2.fastq.gz	Fastqsanger.gz
	SRR8607562_1.fastq.gz	Fastqsanger.gz

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Transcriptome Analysis of the different expressed genes affected by ClpP in B. abortus 2308

Project description:Purpose:To gain insight into the spectrum of Brucella gene affected by the ClpP protease, as well as the genetic basis for the distinctive phenotypic properties exhibited by the clpP mutant strain. Methods: Three B. abortus 2308 and the three clpP mutant were grown in TSB at 37℃ until the log phase was reached, total RNA was isolated using the TRIzol according to the manufacturer’s instructions.The sequencing library of each RNA sample was prepared by using NEB Next Ultra Directional RNA Library Prep Kit for Illumina as recommended by the manufacturer.The HiSeq 4000 platform was used to perform the transcriptome sequencing. Results: In total, 772 genes that exhibited different expression (Fold change>4, and FDR<0.05) were identified in the clpP mutant. In the clpP mutant strain, 376 genes were up-regulated and 396 genes were down-regulated. Conclusions: According to the result of COG analysis, the different expressed genes were mainly involved in Energy production and conversion, Cell wall/membrane/envelope biogenesis, and Carbohydrate transport and metabolism. KEGG pathway analysis demonstrated that the genes exhibited significant difference were primarily enriched in ABC transporters, beta-Lactam resistance, Oxidative phosphorylation, Cationic antimicrobial peptide (CAMP) resistance, Limonene and pinene degradation, Lysine degradation, Valine, leucine and isoleucine degradation, Chloroalkane and chloroalkene degradation, Ascorbate and aldarate metabolism, Tryptophan metabolism, Citrate cycle (TCA cycle), and Histidine metabolism.

2022-02-22 | GSE126915 | GEO

Transcription profiling of B. abortus BvrS BvrR from B. abortus 2308

Project description:The analysis of the expression profile of the two component system BvrR/BvrS of the B. abortus 2308 making the comparison of the expression of the B. abortus 2308 and B. abortus 2308 BvrR- (mutant in the gen BvrR) whit the microarray of brucella melitensis (Brucearray)

2010-04-22 | E-MEXP-2564 | biostudies-arrayexpress

Expression Data from Brucella abortus strain 2308 and isogenic mavR mutant cells

Project description:MavR is a 160 nucleotide regulatory RNA molecule that is produced during B. abortus strain 2308 growth in nutrient-replete broth. We used custom-made Affymetrix B. abortus strain 2308 derived GeneChips to compare the gene expression properties of wild type and isogenic mavR mutant cells.

2023-11-20 | GSE247672 | GEO

Expression Data from Brucella abortus strain 2308 and isogenic vtlR mutant cells

2015-08-03 | GSE69564 | GEO

Expression Data from Brucella abortus strain 2308 and isogenic vtlR mutant cells

Project description:VtlR (virulence-associated transcriptional LysR-family regulator) is thought to be a transcriptional regulator of B. abortus virulence determinants We used custom-made Affymetrix B. abortus strain 2308 derived GeneChips to compare the gene expression properties of wild type and isogenic vtlR mutant cells. B. abortus strain 2308 or vltR mutant cells were grown to late exponential phase growth, total bacterial RNA was isolated and subjected to GeneChip hybridization and analysis. We sought to determine the regulatory effects of VtlR.

2015-08-03 | E-GEOD-69564 | biostudies-arrayexpress

Expression Data from Brucella abortus strain 2308 and isogenic mucR mutant cells

Project description:MucR is one of the few transcriptional regulatory proteins that has been linked to Brucella pathogenesis. We used custom-made Affymetrix B. abortus strain 2308 derived GeneChips to copare the gene expression properties of wild type and isogenic mucR mutant cells. B. abortus strain 2308 or mucR mutant cells were grown to stationary phase growth, total bacterial RNA was isolated and subjected to GeneChip hybridization and analysis. We sought to determine the regulatory effects of MucR.

2012-12-01 | E-GEOD-40532 | biostudies-arrayexpress

Expression Data from Brucella abortus strain 2308 and isogenic mucR mutant cells

2012-12-01 | GSE40532 | GEO

Brucella abortus 2308

Project description:Brucella abortus 2308 Raw sequence reads

| PRJNA765312 | ENA

Expression Data from Brucella abortus strain 2308 and isogenic abcR1 abcR2 mutant cells

Project description:abcR1 and abcR2 produce two regulatory RNA molecules, that are produced during stationary phase growth. We used custom-made Affymetrix B. abortus strain 2308 derived GeneChips to compare the gene expression properties of wild type and isogenic abcR1 abcR2 double mutant cells. B. abortus strain 2308 or abcR1 abcR2 double mutant cells were grown to stationary phase growth, total bacterial RNA was isolated and subjected to GeneChip hybridization and analysis. We sought to determine the regulatory effects of abcR1 and abcR2 RNA species.

2012-06-14 | E-GEOD-38017 | biostudies-arrayexpress

Expression Data from Brucella abortus strain 2308 and isogenic abcR1 abcR2 mutant cells

2012-06-15 | GSE38017 | GEO