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Single cell RNA-seq analysis of hair cell regeneration in the mammalian vestibular system and its potentiation by Atoh1

ABSTRACT: Single cell RNA-seq analysis of hair cell regeneration in the mammalian vestibular system and its potentiation by Atoh1

PROVIDER: PRJNA525021 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8649370_1.fastq.gz	Fastqsanger.gz
	SRR8649370_2.fastq.gz	Fastqsanger.gz
	SRR8649380_1.fastq.gz	Fastqsanger.gz
	SRR8649380_2.fastq.gz	Fastqsanger.gz
	SRR8649390_1.fastq.gz	Fastqsanger.gz

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RNA-seq analysis of hair cell regeneration in the mammalian vestibular system and its potentiation by Atoh1

Project description:RNA-seq analysis of hair cell regeneration in the mammalian vestibular system and its potentiation by Atoh1

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Transcriptomic and epigenetic regulation of hair cell regeneration in the mouse utricle and its potentiation by Atoh1

Project description:The mammalian cochlea loses its ability to regenerate new hair cells prior to the onset of hearing. In contrast, the adult vestibular system can produce new hair cells in response to damage, or by reprogramming of supporting cells with the hair cell transcription factor Atoh1. We used RNA-seq and ATAC-seq to probe the transcriptional and epigenetic responses of utricle supporting cells to damage and Atoh1 transduction. We show that the improved regenerative response of the utricle correlates with a more accessible chromatin structure in utricle supporting cells compared to their cochlear counterparts. We also provide evidence that Atoh1 transduction of supporting cells is able to promote increased transcriptional accessibility of some hair cell genes. Our study offers a possible explanation for regenerative differences between sensory organs of the inner ear, but shows that additional factors to Atoh1 may be required for optimal reprogramming of hair cell fate.

2019-04-30 | GSE121610 | GEO

RNA-seq analysis of hair cell regeneration in the mammalian vestibular system and its potentiation by Atoh1

Project description:We used RNA-seq to probe the transcriptional and epigenetic responses of utricle supporting cells to damage and Atoh1 transduction.

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Single cell RNA-seq analysis of hair cell regeneration in the mammalian vestibular system and its potentiation by Atoh1

Project description:We used single cell RNA-seq to probe the transcriptional responses of utricle supporting cells to damage and Atoh1 transduction.

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In Vivo and Ex Vivo Utricles treated with ATOH1 under different promoters

Project description:Vestibular hair cells are mechanoreceptors critical for detecting head position and motion. In mammals, hair cell loss causes vestibular dysfunction as spontaneous regeneration is nearly absent. Constitutive expression of exogenous ATOH1, a hair cell transcription factor, increases regeneration of hair cells, but these cells fail to mature. With the aim of developing an optimized AAV-based ATOH1 gene therapy, we used single-cell RNA-Seq and explored the impact of defined promoters on ATOH1 transgene expression level, timing, effects in distinct cell types, and the maturity of regenerated vestibular hair cells ex vivo and in vivo.

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Regenerating hair cells in human vestibular sensory epithelia

Project description:To investigate alterations in gene expression after Ad2-ATOH1-GFP transduction of human supporting cells, we measured transcriptome changes by RNA-seq. We compared gentamicin-treated tissue (to kill the hair cells) transduced with Ad2-ATOH1-GFP (to promote hair cell production) cultured for 5 days, versus control tissues only treated with gentamicin (cultured for 2 days, 8 days and 14-18 days).

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Project description:Transcriptomic and epigenetic regulation of hair cell regeneration in the mouse utricle and its potentiation by Atoh1

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