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Small RNA sequencing of bone marrow extracellular vesicle labeled Granulocyte macrophage progenitors (GMPs) [GMP.sRNA]

ABSTRACT: Small RNA sequencing of bone marrow extracellular vesicle labeled Granulocyte macrophage progenitors (GMPs) [GMP.sRNA]

PROVIDER: PRJNA525665 | ENA |

REPOSITORIES: ENA

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Extracellular vesicle labeled Granulocyte macrophage progenitors

Project description:Extracellular vesicle labeled Granulocyte macrophage progenitors

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Gene expression by RNA sequenicng of extracellular vesicle labeled Granulocyte macrophage progenitors (GMPs) [mRNA]

Project description:Gene expression by RNA sequenicng of extracellular vesicle labeled Granulocyte macrophage progenitors (GMPs) [mRNA]

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Small RNA sequencing of bone marrow extracellular vesicle labeled Granulocyte macrophage progenitors (GMPs) [GMP.sRNA]

Project description:Small RNA sequenicg was performed on mouse bone marrow GMPs (Lin-, cKit+, Sca1-, CD34+, CD16/32 hi) labeled by osteoblastic cell derived extracellular vesicles as indicated by GFP labeling. Cells were isolated by FACS sorting from the bone marrow of Ocn-GFPTopaz mice and total RNA was extracted using the miRNeasy micro kit from (Qiagen)

2021-12-08 | GSE127869 | GEO

Gene expression by RNA sequenicng of extracellular vesicle labeled Granulocyte macrophage progenitors (GMPs) [mRNA]

Project description:RNA sequenicg was performed on mouse bone marrow GMPs (Lin-, cKit+, Sca1-, CD34+, CD16/32 hi) labeled by osteoblastic cell derived extracellular vesicles as indicated by GFP labeling. Cells were isolated by FACS sorting from the bone marrow of Ocn-GFPTopaz mice and total RNA was extracted using the miRNeasy micro kit from (Qiagen)

2021-12-08 | GSE127858 | GEO

Extracellular vesicle labeled Granulocyte macrophage progenitors

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-12-08 | GSE127872 | GEO

RNA-seq of mouse myeloid progenitors reveals two independent pathways for monocyte production via GMPs and MDPs

Project description:Granulocyte-monocyte progenitors (GMPs) and monocyte-dendritic cell progenitors (MDPs) produce monocytes during homeostasis and in response to increased demand during infection. Both progenitor populations are thought to derive from common myeloid progenitors (CMPs), and a hierarchical relationship (CMP-GMP-MDP-monocyte) is presumed to underlie monocyte differentiation. Here, however, we demonstrate that mouse MDPs arose from CMPs independently of GMPs, and that GMPs and MDPs produced monocytes via similar, but distinct, monocyte-committed progenitors. GMPs and MDPs yielded classical (Ly6Chi) monocytes with gene expression signatures that were defined by their origins and impacted their function. GMPs produced a subset of “neutrophil-like” monocytes, whereas MDPs gave rise to a subset of monocytes that yielded monocyte-derived dendritic cells. GMPs and MDPs were also independently mobilized to produce specific combinations of myeloid cell types following the injection of microbial components. Thus, the balance of GMP and MDP differentiation shapes the myeloid cell repertoire during homeostasis and following infection.

2017-11-21 | GSE88982 | GEO

scRNAseq reveals heterogeneity among mouse bone marrow Ly6Chi monocytes produced by GMPs and MDPs

2017-11-13 | GSE104478 | GEO

Effect of Dot1l deletion on LSKs and GMPs

Project description:We wanted to investigate the effects of Dot1l deletion on gene expression in LSKs and GMPs of C57/BL6 mice Aberrant Hox gene activation is a recurrent feature in several different types of human leukemia, including leukemias with rearrangements of the mixed lineage leukemia (MLL) gene. In this study, we demonstrate that Hox gene expression is controlled by higher degree H3K79 methylation in acute myeloid leukemia (AML). We show that the deposition of progressive H3K79 methylation states at the genomic loci of critical Hox genes is dependent on the interaction of the H3K79 methyltransferase Dot1l with Af10, a protein that is found in the Dot1l complex isolated from diverse cell types. Furthermore, abrogation of the Dot1l-Af10 interaction reverses aberrant epigenetic profiles found in the leukemia epigenome and impairs the transforming ability of mechanistically distinct AML oncogenes. Lineage negative Sca-1 positive Kit positive (LSK) cells and granulocyte macrophage progenitors (GMPs) were sorted from Dot1 wt/wt x Mx-Cre mice or Dot1l fl/fl x Mx-Cre mice were injected with PIPC. PIPC injection induced biallelic deletion of the Dot1l allele in the Dot1l fl/fl mice but not the Dot1l wt/wt mice. The Dot1l wt/wt LSKs and GMPs were compared to the Dot1l -/- counterparts by RNA extraction and Microarrays.

2014-09-08 | E-GEOD-54498 | biostudies-arrayexpress

Microarray data from L-GMPs and GMPs

Project description:A leukemia cell fraction highly enriched for LSCs was generated in a mouse model of AML induced by co-expression of MLL target genes Hoxa9 and Meis1. Limit dilution transplantation analyses performed on various prospectively isolated leukemia cell subpopulations revealed that cells capable of transplanting AML to syngeneic recipient mice (the operational definition of LSCs) were highly enriched in the leukemia cell fraction displaying an immunophenotype (Lin- Sca1- c-kit+ CD16/32+ CD34+) comparable to normal GMPs, referred to as L-GMPs. For the purpose of identifying genes that are differentially expressed in LSCs, microarray expression profiling was performed on L-GMPs (from leukemic mice) and GMPs (from normal mouse BM) purified by flow cytometry.

2015-10-02 | GSE64773 | GEO

Caenorhabditis elegans extracellular vesicle

Project description:Caenorhabditis elegans extracellular vesicle

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