Project description:Genome-scale DNA methylation profiling using the Infinium DNA methylation 450K BeadChip platform and samples from gastric cancer (intestinal and diffuse), precursor lesions (multifocal chronic atrophic gastritis and inestina metaplasia), non-atrophic gastritis and normal gastric mucosa.
Project description:We performed genome-wide DNA methylation profiling of precursor lesions of endometrial endometrioid carcinoma tissues derived from patients in the Cancer Institute Hospital of Japanese Foundation for Cancer Research.
Project description:Background:
Gastric cancer is a leading cause of cancer deaths around the world. This disease is a serious problem in places like East Asia, Central and South America, and Eastern Europe. Researchers want to study the causes of gastric cancer and its precursors. They want to reduce the number of people with stomach cancer.
Objectives:
To learn more about bacteria factors and other causes of gastric cancer. To study potential markers associated with precancerous gastric lesions (intestinal metaplasia).
Eligibility:
Adults ages 40-70 years at certain hospitals in Chile who:
Are going to have upper gastrointestinal endoscopies
OR have stomach cancer and need surgery
Design:
Participants will give gastric tissue samples.
Some participants will donate a portion of the stomach tissue that is removed as part of their clinical care.
Participants will give access to reports of their stomach exam. They will allow researchers to photograph the microscope slides of their tissue samples.
Participants will answer questions. The topics of the questions include:
Age, height, weight
Education
Habits including tobacco and alcohol
Personal and family history of disease
Reproductive history
Diet
Some participants will give blood, urine, saliva, and stool samples. Study staff will collect the blood. They will tell the participants how to collect the other samples themselves.
Project description:The association between lifestyle and dietary pattern with precursor lesions of colorectal cancer is still unclear. This study is conduced in Jinhua, a city with a high prevalence of colorectal cancer in China, to assess relations between precursor lesions of colorectal cancer and lifestyle and dietary factors.
Project description:Genome wide DNA methylation profiling of normal gastric epuithelial cells and gastric cancer cell lines. The Illumina Infinium 450k Human DNA methylation Beadchip v1.2 was used to obtain DNA methylation profiles across approximately 450,000 CpGs. Samples included 2 normal gastric epithelial cells and 14 gastric cancer cells.
Project description:Time-series comprehensive DNA methylation analysis after EBV infection in the immortalised normal gastric epithelial cell line, GES1, and low methylation gastric cancer cell line, MKN7. Infinium HumanMethylation450 BeadChip was used to obtain DNA methylation profiles across 485,577 CpG sites. Samples included 9 GES1 with and without EBV infection, 5 MKN7 with and without EBV infection, EBV+ gastric cancer cell and xenograft, SNU719 and KT, 2 normal gastric mucosae, 2 low-methylation gastric cancer cases, and 2 high-methylation gastric cancer cases.
Project description:A detailed understanding of the molecular alterations in the course of cholangiocarcinogenesis is crucial for a better understanding of this disease and may pave the way to early diagnosis and better treatment options for cholangiocarcinoma (CCA) patients. We analyzed a clinicopathologically well-characterized patient cohort with intraductal papillary (IPNB) or tubulopapillary biliary (ITPN) precursor lesions of the biliary tract. For a large subset of patients, the triplet sample set of non-neoplastic biliary epithelium, IPNB or ITPN, and invasive CCA was available.