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ABSTRACT: Mayaro virus sequencing

PROVIDER: PRJNA525867 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8690440.fastq.gz	Fastqsanger.gz
	SRR8690440_1.fastq.gz	Fastqsanger.gz
	SRR8690440_2.fastq.gz	Fastqsanger.gz
	SRR8690441.fastq.gz	Fastqsanger.gz
	SRR8690441_1.fastq.gz	Fastqsanger.gz

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Alterations in the metabolic footprint induced by Mayaro virus infection in cell culture

Project description:This study focuses metabolic footprinting of Vero cells infected by Mayaro virus. Nuclear magnetic resonance combined with multivariate analytical methods and tools of pattern recognition shows that metabolic changes can be attributed to the effect of Mayaro virus infection. Vero cells infected by Mayaro and incubated for periods of 2h, 6h and 12h show in each period different variations in the levels of several metabolites such as amino acids, organic acids and carbohydrate. These organic compounds are metabolites involved in the pathway of glycolysis, tricarboxylic acid cycle, pentose phosphate pathway, and the oxidation pathway of fatty acids (via the β-oxidation). This study of footprinting analysis demonstrates the effect of the action of virus in the metabolism of the Vero cells, moreover, these analyses indicate intracellular metabolic state, and contributes to the knowledge of the microorganism influence on cellular metabolism.

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Project description:Mayaro virus (MAYV) is a mosquito-borne Alphavirus responsible for outbreaks in South America and the Caribbean. In this study we infected Anopheles stephensi with MAYV and sequenced mRNA and small RNA to understand how MAYV infection impacts gene transcription and the expression of small RNAs in the mosquito vector. Genes involved with innate immunity and autophagy are regulated in response to MAYV infection of An. stephensi, we also discover novel miRNAs and describe their expression patterns following bloodmeal ingestion. These results suggest that MAYV does induce a molecular response to infection in its mosquito vector species and that MAYV may have mechanisms to evade the vector immune response.

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Project description:Mayaro Virus as the cause of Acute Febril Illness in the Colombian Amazon Basin

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