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Changes in the biologica function of necroptotic, peripheral blood mononuclear cells under irradiation in vitro

ABSTRACT: Changes in the biologica function of necroptotic, peripheral blood mononuclear cells under irradiation in vitro

PROVIDER: PRJNA525984 | ENA |

REPOSITORIES: ENA

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Changes in the biologica function of necroptotic, peripheral blood mononuclear cells under irradiation in vitro

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Project description:Ionizing Radition is known to cause cell damage. Human peripheral blood mononuclear cells have long been used to study radiation induced gene expression profiling. Within this study we evaluated gene and microRNA expression alterations of human PBMC irradiated with 60 Gy g-ray. Cells were cultured for 2, 4 and 20h after irradiation before RNA was isolated and Agilent whole human GenomeOligo Microarray was performed. Radiation induced gene expression in human peripheral blood mononuclear cells were measured at 2, 4 and 20 hours after exposure to doses of 60 Gy g-rays. Non radiated cell of each donor and time point were used as contol cells. Four independent experiments were performed at each time (2, 4, or 20 hours and 0 h - only 1 sample per donor) using 4 different donors.

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