Project description:We deep sequenced and analyzed miRNAs using deep RNA sequencing (RNA-seq) in cage rearing and traditional breeding duck's duodenum sample of Nonghu NO.2 duck. 21 differentially expressed miRNA were identified in the duodenum. 6 miRNAs were upregulated and 15 were downregulated in the cage rearing duck's duodenum of the Nonghu NO.2 duck compared to their expression in the control group. These findings provided insights into the expression profiles of miRNAs in duck duodenum, and deepened our understanding of miRNAs in oxidative injury of duck.
Project description:The objective of this study was to identify candidate circular RNAs associated with duck muscle development. circRNA sequencing analysis was employed using female breast muscle samples embryo stage 13th day (E13) and embryo stage 19th day (E19). RNA-seq data and validation experiment in duck myoblast cells showed that circGAS2-2 significantly differential expressed between E13 and E19 group.
Project description:We deep sequenced and analyzed miRNAs using deep RNA sequencing (RNA-seq) in transported and control duck's duodenum sample of Jingjiang duck. We analyzed the miRNA data with 9467248 reads and 9808143 million reads, obtained 9338224 and 9677777 clean reads in transported and control duck's by high-throughput sequencing, respectively. we respectively gained 4636135 and 4759049 miRNAs sequences in two groups by filtering the known non-miRNA reads, such as rRNA, tRNA, snRNA, and snoRNA by screening against ncRNA deposited in the GenBank and Rfam databases. These findings provided insights into the expression profiles of miRNAs in duck duodenum, and deepened our understanding of miRNAs in transportation injury of duck.
Project description:Purpose: To study the expression profile of piRNAs in rat ovary Methods: Ovary from eight-weeks old, adult wistar rats were harvested after sacrificing the animal. The total RNA is isolated using trizol reagent. Subsequently, small RNA library was contructed using illumina kit as per manufacturer's instructions. Results: The reads were annotated to the rat genome (rn5) and piRNA database and their expression is studied Conclusions: Large number of piRNAs are expressed in rat ovary
