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ABSTRACT: S.aureus USA300 LAC Vancomycin

PROVIDER: PRJNA526539 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8710730_1.fastq.gz	Fastqsanger.gz
	SRR8710730_2.fastq.gz	Fastqsanger.gz
	SRR8710731_1.fastq.gz	Fastqsanger.gz
	SRR8710731_2.fastq.gz	Fastqsanger.gz
	SRR8710732_1.fastq.gz	Fastqsanger.gz

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Expression analysis of Staphylococcus aureus USA300 LAC in response to vehicle and benzbromarone.

Project description:Compilation fo whole genome gene expression changes in Staphylococcus aureus USA300 LAC cultures grown in the presence of vehicle or the anti-gout drug benzbromarone. The drug was intially screened as effective against the agr quorum sensing system in Staphylococcus aureus AH1677. A microarray study using total RNA harvested from three cultures of Staphylococcus aureus USA300 LAC plus vehicle control and three cultures of Staphylococcus aureus USA300 LAC plus 12 uM benzbromarone.

2014-03-19 | E-GEOD-55980 | biostudies-arrayexpress

Prophage-encoded methyltransferase upregulates virulence to drive adaptation in an outbreak of community-acquired methicillin-resistant Staphylococcus aureus [Harm analysis]

Project description:To determine the effects of phage m⏀11 on gene expression in USA300 (LAC*)

2024-04-17 | GSE255351 | GEO

Prophage-encoded methyltransferase upregulates virulence to drive adaptation in an outbreak of community-acquired methicillin-resistant Staphylococcus aureus

Project description:To determine the effects of phage adenine methyltransferase (pamA) on gene expression in WT USA300 (LAC*)

2024-04-17 | GSE252862 | GEO

RNA-Seq based comparison of Staphylococcus aureus strains resistent and sensitive to MT02

Project description:A MT02 sensitive and resistant strain of Staphylococcus LAC USA300 JE2 were exposed to MT02. From those cell RNA was extracted and anylsed by RNA-Seq

2017-11-05 | GSE87572 | GEO

Global changes in Staphylococcus aureus gene expression in human blood provide insight into mechanisms of immune evasion and virulence

Project description:Staphylococcus aureus is a leading cause of bloodstream infections worldwide. In the United States, many of these infections are caused by a strain known as USA300. Although progress has been made, our understanding of the S. aureus molecules that promote bacteremia and survival in human blood is incomplete. To that end, we analyzed the USA300 transcriptome during culture in human blood, human serum, and trypticase soy broth (TSB), a standard laboratory culture media. Notably, genes encoding several cytolytic toxins were up-regulated in human blood over time, and hlgA, hlgB, and hlgC (encoding gamma-hemolysin subunits HlgA, HlgB, and HlgC) were among the most highly up-regulated genes at all time points. Culture supernatants derived from a USA300 isogenic hlgABC-deletion strain (LAC?hlgABC) had significantly reduced capacity to form pores in human neutrophils and ultimately cause neutrophil lysis. Compared with the wild-type USA300 strain (LAC), LAC?hlgABC had modestly reduced ability to cause mortality in a mouse bacteremia model. On the other hand, wild-type and LAC?hlgABC strains caused virtually identical disease in a mouse skin infection model, and bacterial survival and neutrophil lysis after phagocytosis in vitro was similar between these strains. Comparison of the cytolytic capacity of culture supernatants from wild-type and isogenic deletion strains lacking hlgABC, lukS/F-PV (encoding PVL), and/or lukDE revealed significant functional redundancy among two-component leukotoxins in vitro. These findings may explain in part the apparent limited contribution of any single two-component leukotoxin to USA300 immune evasion and virulence. S. aureus strain USA300 transcriptome during culture in human blood, human serum, and trypticase soy broth (TSB): time course.

2011-08-25 | E-GEOD-25454 | biostudies-arrayexpress

Genomic and Long-Term Transcriptomic Imprints Related to the Daptomycin Mechanism of Action Occurring in Daptomycin- and Methicillin-Resistant Staphylococcus aureus Under Daptomycin Exposure

Project description:Daptomycin (DAP) is the last-resort treatment for heterogeneous Vancomycin-Intermediate-Staphylococcus aureus (hVISA) and Vancomycin-Intermediate-S.aureus (VISA), and DAP-resistance onset which is also linked to reduced vancomycin susceptibility, is an increasing public health problem. To have more insight into the mechanisms of daptomycin resistance, the comparative transcriptomes of two DAP-R (1C-3B) clinical isogenic isolates vs their DAP-S (1A-3A) counterparts were investigated by Illumina RNA-seq, the Rockhopper tool, computational filtering analyses and bioinformatic tools.

2020-08-07 | GSE121797 | GEO

Expression analysis of Staphylococcus aureus USA300 LAC in response to vehicle and benzbromarone.

2014-03-19 | GSE55980 | GEO

NarGHJI mediated virulence in Staphylococcus aureus

Project description:To investigate the function NarGHJI in the regulation of the expression of virulence factors, we constructed a narGHJI mutant by targeted deletion of narG in MRSA isolate USA300 LAC We then performed gene expression profiling analysis using data obtained from RNA-seq of of two different strains at early exponential (OD600 = 0.5).

2022-12-17 | GSE220798 | GEO

The iron starvation response of the Staphylococcus aureus hemB small colony variant (SCV)

Project description:Respiration deficient S. aureus small colony variants (SCVs) frequently cause persistent infections, which necessitates they acquire iron, yet how SCVs obtain iron remains unknown. To address this, we created a stable hemB mutant in S. aureus USA300 strain LAC. The hemB SCV utilized exogenously supplied heme but was attenuated for growth under conditions of iron starvation. RNA-seq showed that both WT S. aureus and the hemB mutant sense and respond to iron starvation.

2022-09-06 | GSE184082 | GEO

Project description:Staphylococcus aureus USA300 LAC Valine Suppressor mutant project

| PRJNA422577 | ENA