Quantitative MNase-seq accurately maps nucleosome occupancy levels
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ABSTRACT:
PROVIDER: PRJNA528497 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR8766790_1.fastq.gz | Fastqsanger.gz | |||
| SRR8766790_2.fastq.gz | Fastqsanger.gz | |||
| SRR8766800_1.fastq.gz | Fastqsanger.gz | |||
| SRR8766800_2.fastq.gz | Fastqsanger.gz | |||
| SRR8766791_1.fastq.gz | Fastqsanger.gz |
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Project description:Micrococcal nuclease (MNase) is widely used to map nucleosomes. However, its aggressive endo-/exo-nuclease activities make MNase-seq unreliable for determining nucleosome occupancies, because cleavages within linker regions produce oligo- and mono-nucleosomes, whereas cleavages within nucleosomes destroy them. Here, we introduce a theoretical framework for predicting nucleosome occupancies and an experimental protocol with appropriate spike-in normalization that confirms our theory and provides accurate occupancy levels over an MNase digestion time course. As with human cells, we observe no overall differences in nucleosome occupancies between Drosophila euchromatin and heterochromatin, which implies that heterochromatic compaction does not reduce MNase accessibility of linker DNA.
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