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Light induced injury in mouse embryos revealed by single-cell RNA sequencing

ABSTRACT: Light induced injury in mouse embryos revealed by single-cell RNA sequencing

PROVIDER: PRJNA528503 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8767100_1.fastq.gz	Fastqsanger.gz
	SRR8767100_2.fastq.gz	Fastqsanger.gz
	SRR8767110_1.fastq.gz	Fastqsanger.gz
	SRR8767110_2.fastq.gz	Fastqsanger.gz
	SRR8767120_1.fastq.gz	Fastqsanger.gz

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Light induced injury in mouse embryos revealed by single-cell RNA sequencing

Project description:We adopt mouse pre-implantation embryos to evaluate the effects of different exposure time and illumination intensity. The damage-causing time and intensity is chosen to treat embryos at 2-cell stage. Single-cell transcriptomes from 2-cell to blastocyst stage are analyzed under both control and high-intensity light conditions. These data show that embryo cells at each embryonic stage can be categorized into different light conditions. Further analyses of differentially expressed genes and relative GO terms reveal the light-induced injury as well as the potential repair response after high-intensity illumination treatment. Maternal-to-zygote transition is also affected through the failure of removing maternal RNAs and deactivating zygotic genomes. In conclusion, our work revealed integrated response of high-intensity light, involving the long-lasting injury effects, damage repair mechanism and blastocyst morphology changes.

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Light-induced injury in mouse embryos revealed by single-cell RNA sequencing.

Project description:BackgroundLight exposure is a common stress factor in in vitro manipulation of embryos in the reproductive center. Many studies have shown the deleterious effects of high-intensity light exposure in different animal embryos. However, no transcriptomic studies have explored the light-induced injury and response in preimplantation embryos.ResultsHere, we adopt different time-courses and illumination intensities to treat mouse embryos at the 2-cell stage and evaluate their effects on blastulation. Meanwhile, single-cell transcriptomes from the 2-cell to blastocyst stage were analyzed after high-intensity light exposure. These data show that cells at each embryonic stage can be categorized into different light conditions. Further analyses of differentially expressed genes and GO terms revealed the light-induced injury as well as the potential repair response after high-intensity lighting. Maternal-to-zygote transition is also affected by the failure to remove maternal RNAs and deactivate zygotic genome expression.ConclusionOur work revealed an integrated response to high-intensity lighting, involving morphological changes, long-lasting injury effects, and intracellular damage repair mechanisms.

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