Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Knock-down of all HIF3A splice variants under 1 % hypoxia by siRNA targeting a shared region in Hep3B cells

ABSTRACT: Knock-down of all HIF3A splice variants under 1 % hypoxia by siRNA targeting a shared region in Hep3B cells

PROVIDER: PRJNA529071 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Knock-down of all HIF3A splice variants under 1 % hypoxia by siRNA targeting a shared region in Hep3B cells

Project description:The human HIF3A gene undergoes extensive alternative splicing resulting in seven known isoforms. The short splice variant, HIF-3alpha4, is an inhibitor of the hypoxia response driven by HIF-1 and HIF-2. The role of the long isoforms remains unclear. We used cDNA microarray to study the overall impact of HIF3A knock-down by transfecting Hep3B cells with siRNA targeting a region shared by all splice variants under 1 % hypoxia.

2019-12-31 | GSE128846 | GEO

Splicing of polyA transcripts in Hep3B cells

Project description:We were interested in identifying novel splice junctions in Hep3B cells, especially in genes involved in cholesterol metabolism like HMGCR, so we sequenced polyA-tailed RNA from Hep3B cells. 1 polyA-selected RNA sample was sequenced from Hep3B cells

2014-05-15 | E-GEOD-57666 | biostudies-arrayexpress

Splicing of polyA transcripts in Hep3B cells

2014-05-15 | GSE57666 | GEO

Overexpression of the long Hypoxia inducible factor (HIF)-3a2 splice variant under 1 % hypoxia in Hep3B cells

Project description:The human HIF3A gene undergoes extensive alternative splicing resulting in seven known isoforms. The short splice variant, HIF-3alpha4, is an inhibitor of the hypoxia response driven by HIF-1 and HIF-2. The role of the long isoforms remains unclear. We used cDNA microarray to study the overall impact of HIF-3a2 overexpression in Hep3B cells under 1 % hypoxia.

2019-12-31 | GSE128845 | GEO

Differential gene expression in Hep3B and Hep3B SOX4-/- cells

Project description:Overexpression of SOX4 in various kinds of cancers specimen was associated with poor prognosis of patients; however, the role of SOX4 in angiogenesis or tumor microenvironment modulation remains unclear. Therefore the endogenous SOX4 was knockout and the differential gene expression between Hep3B and Hep3B SOX4-/- cells were examined via genechip. We found that the differentially expressed genes, EzH2, a SOX4-associated partner, and CXCL12, were repressed in Hep3B SOX4-/- cells compared with parental Hep3B; these results were further assessed via qRT-PCR in Hep3B SOX4-/- versus Hep3B cells.

2020-12-01 | GSE141909 | GEO

Effect of depletion of CPSF6 on gene expression of Hep3B hepatocellular carcinoma cells

Project description:To investigate the associated pathway of CSPF6, we established a hep3B cell line in which the target gene was broken down by siRNA.

2024-04-07 | GSE229281 | GEO

Transcriptomes Sequencing of si-EIF4G2 and si-NC groups in Hep3B cells

Project description:We transfected siRNA-EIF4G2 and si-NC into Hep3B cells to explore the differentially expressed genes in cells and the affected intracellular signaling pathways after EIF4G2 knockdown

2023-12-14 | GSE163180 | GEO

Differential gene expression in Hep3B and Hep3B SOX4-/- cells

Project description:Differential gene expression in Hep3B and Hep3B SOX4-/- cells

| PRJNA595153 | ENA

RNA-Seq in Hep3B-shNC and Hep3B-shlnc-CTHCC cells

Project description:RNA-Seq in Hep3B-shNC and Hep3B-shlnc-CTHCC cells

| PRJNA674827 | ENA

Overexpression of the long Hypoxia inducible factor (HIF)-3a2 splice variant under 1 % hypoxia in Hep3B cells

Project description:Overexpression of the long Hypoxia inducible factor (HIF)-3a2 splice variant under 1 % hypoxia in Hep3B cells

| PRJNA529070 | ENA