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Cdx2 ChIP-seq performed in E8.5 whole mouse embryos

ABSTRACT: Cdx2 ChIP-seq performed in E8.5 whole mouse embryos

PROVIDER: PRJNA529107 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8786621_1.fastq.gz	Fastqsanger.gz
	SRR8786621_2.fastq.gz	Fastqsanger.gz
	SRR8786622_1.fastq.gz	Fastqsanger.gz
	SRR8786622_2.fastq.gz	Fastqsanger.gz

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Cdx2 ChIP-seq performed in E8.5 whole mouse embryos

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Project description:The object of this study was to identify genes transcriptionally upregulated and downregulated in response to Tcof1 haploin-sufficiency during mouse embryogensis Experiment Overall Design: Total RNA was extracted from 3 E8.5 wild-type and 3 E8.5 Tcof1+/- littermate embryos using the RNeasy Mini Protocol for Isolation of Total RNA from Animal Tissues (Qiagen) according to the manufacturerâs protocol. RNA quality and quantity was determined using the Bioanalyser. To generate targets for microarray analysis, total RNA (100 ng) from each wild-type and mutant embryo was amplified using Two-Cycle Target Labeling (Affymetrix) according to the manufacturerâs instructions. Biotinylated target cRNAs (20 Î¼g) from the 3 wild-type and 3 Tcof1+/- embryos were hybridised to separate GeneChipÂ® Mouse Genome 430 2.0 arrays (Affymetrix), following standard Affymetrix procedures.

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