Project description:The endocrine changes and gene regulation patterns responding to sexual maturation and spawning have not been clarified in fish species. To identify the potential metabolites and genes regulating the ovarian development and spawning, we employed the approach integrating the metabolic and RNA-seq techniques to investigate the metabolites and candidate genes participating in the sexual maturation and spawning of female blunt snout bream Megalobrama amblycephala. This data descriptor provides metabolic and transcriptomic information for the blood tissue at different developmental stages of female M. amblycephala. With UPLC-MS/MS method, a total of 763 and 173 differential ions were detected from the pairwise comparisons of the four groups in positive and negative mode, respectively. Meanwhile, 191.65 Mb raw reads were generated using illumina Hiseq platform with 50 bp single-end strategy. We then analysed the differential metabolites and differentially expressed genes and their functions among the four groups. This data descriptor will contribute to understanding the dynamics of metabolites and gene regulation in ovarian development and spawning and reuse to support physiological and genetic researches in aquaculture.
Project description:Identification and characterization of microRNAs involved in growth of blunt snout bream (Megalobrama amblycephala) by Solexa sequencing
Project description:The present study provides the first large-scale characterization of miRNAs in M. amblycephala and miRNA profile of M. amblycephala with different growth performance. The discovery of miRNA resource from this study is expected to contribute to a better understanding of the miRNAs roles playing in regulating the growth biological processes in fish and the study of miRNA function and phenotype-associated miRNA identification in fish. To investigate the functions of miRNAs associated with growth of M. amblycephala, we adopted the Solexa sequencing technology to sequence two small RNA libraries prepared from four growth related tissues (brain, pituitary, liver and muscle) of M. amblycephala using individuals with relatively high and low growth rates.
Project description:Unveiling the roles of metabolites and gene regulation in sexual maturation and spawning of blunt snout bream (Megalobrama amblycephala)