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Directed differentiation to nephron progenitor cells in the presence and absence of glutamine [RNA Seq 2]

ABSTRACT: Directed differentiation to nephron progenitor cells in the presence and absence of glutamine [RNA Seq 2]

PROVIDER: PRJNA529421 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8792984.fastq.gz	Fastqsanger.gz
	SRR8792985.fastq.gz	Fastqsanger.gz
	SRR8792986.fastq.gz	Fastqsanger.gz
	SRR8792987.fastq.gz	Fastqsanger.gz
	SRR8792988.fastq.gz	Fastqsanger.gz

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Similar Datasets

Directed differentiation to nephron progenitor cells in the presence and absence of glutamine [RNA Seq 2]

Project description:Metabolic pathways driving differentiation into nephron progenitor cells (NPCs) and renal organoids from the pluripotency stage remain poorly understood. In this study, we systematically performed comprehensive metabolite and transcriptome profiling of human pluripotent stem cells (hPSCs) during differentiation into NPCs and further into multicellular organoids. We found activation of distinct metabolic pathways during early-stage progression from hPSCs to NPCs; however, later-stage differentiation from NPCs to organoids, and the intervening developmental stages between them, largely shared similar metabolic profiles. Among the pathways changing in early differentiation, the alanine-aspartate-glutamate and glutamine pathways were found to be significantly altered by both an enrichment and by pathway impact analysis. Moreover, hPSCs survived glutamine deprivation during in vitro differentiation, and NPCs were successfully generated both in the absence and presence of glutamine and glutamate. Surprisingly, glutamine deprivation resulted in enhanced maturation, by accelerating PAX8 expression, and enriching for podocytes as detected through single cell RNA-Seq analysis. Taken together, these findings highlight a critical regulatory role of glutamine metabolism in the derivation of nephron progenitor cells and renal organoids and identify a controlling metabolic pathway of early renal differentiation.

2022-06-01 | GSE128938 | GEO

Directed differentiation to nephron progenitor cells [RNA Seq 1]

2022-06-01 | GSE128937 | GEO

Expression data from TH17 cells cultured in the presence or absence of glutamine

Project description:T cell fate and function is closely related to nutrient uptake and utilization. Here we demonstrate that glutamine deprivation switch Th17 cells to Treg cells in a time and dose dependent manner. The finding highlight the critical role of glutamine in T cell fate determination through a metabolic-epigenetic axis. We performed microarrays to explore the gene expression pattern of TH17 cells in the presence or absence of glutamine.

2024-02-09 | GSE166430 | GEO

Gene expression analysis of H1299 cells in response to the presence or absence of glutamine or glucose

Project description:By a transcriptome analysis using RNA sequencing in H1299 cells, a non-small cell lung cancer cell line (NSCLC), we determined the response of lipogenic genes to absence vs. presence of glutamine (Gln) or glucose (Gluc). We found that neither glutamine nor glucose alone was able to activate the expression of genes regulating fatty acid and cholesterol synthesis, and uptake, including SREBF1, SREBF2, ACLY, ACACA, FASN, SCD1, HMGCR and LDLR, as compared to absence of both. And, activation of lipogenic genes required the presence of both glutamine and glucose, but SCAP gene expression was not affected by either glutamine or glucose. The RNA sequencing analysis in H1299 cells also confirmed that ammonia, similarly to glutamine, significantly activated the expression of genes controlling the fatty acid and cholesterol synthesis pathways as compared to glucose alone. This study detemines the intrinsic molecular connection between glutamine, glucose and lipid synthesis.

2022-04-30 | GSE199089 | GEO

Expression data from TH17 cells cultured in the presence or absence of glutamine

Project description:Expression data from TH17 cells cultured in the presence or absence of glutamine

| PRJNA700974 | ENA

Glutamine deprivation enhances directed differentiation of renal organoids

Project description:Glutamine deprivation enhances directed differentiation of renal organoids

| PRJNA529412 | ENA

Gene expression analysis of H1299 cells in response to the presence or absence of glutamine or glucose

Project description:Gene expression analysis of H1299 cells in response to the presence or absence of glutamine or glucose

| PRJNA818323 | ENA

Directed differentiation to nephron progenitor cells [RNA Seq 1]

Project description:Directed differentiation to nephron progenitor cells [RNA Seq 1]

| PRJNA529420 | ENA

CD4+ T cells in presence or absence of Ikaros

Project description:CD4+ T cells in presence or absence of Ikaros

| PRJNA662885 | ENA

GLS2 links glutamine metabolism and atherosclerosis by remodelling artery walls (aorta).

Project description:Metabolic dysregulation, including perturbed glutamine-glutamate homeostasis, is common in atherosclerotic cardiovascular disease. Here, we reveal that modulation of glutaminolysis or glutamate availability in culture media is critical for smooth muscle cell line (MOVAS) phenotypic switching. Cells were treated for 24 hours with glutaminase inhibitors (50mM DON, Sigma-SML0601) in presence or absence of 2mM glutamate suplementation (Gibco-35050061). High-throughput transcriptional profiling revealed that modulation of glutamine and glutamate homeostasis impacted genes involved in protein and extracellular matrix organization, cell motility and microtubule. Thus, we uncovered a novel role for glutamine metabolism in the phenotypic switch in SMCs, a hallmark of vascular remodelling.

2024-10-17 | GSE270058 | GEO

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