Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from human normal fibroblasts (NFs) and cancer associated fibroblasts (CAFs)

ABSTRACT: Expression data from human normal fibroblasts (NFs) and cancer associated fibroblasts (CAFs)

PROVIDER: PRJNA529597 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Fibroblasts: Normal fibroblasts vs. Carcinoma-associated fibroblasts

Project description:Transcriptional profiling of human carcinoma-associated fibroblasts (CAFs) comparing control normal fibroblasts (NFs). NFs derived from normal tissues and CAFs derived from the patients with oral cancer were identified by immunocytochemistry. Goal was to determine differentially expressed lncRNAs between NFs and CAFs.

2024-01-08 | GSE164473 | GEO

Expression data from (mouse) normal lung fibroblasts and carcinoma-associated fibroblasts

Project description:Cancer-associated fibroblasts (CAFs) have been reported to support tumor progression by a variety of mechanisms. However, their role in the progression of non-small cell lung cancer (NSCLC) remains poorly defined. In addition, the extent to which specific proteins secreted by CAFs contribute directly to tumor growth is unclear. To study the role of CAFs in NSCLC, a cross-species functional characterization of mouse and human lung CAFs was performed, including gene expression analysis comparing normal mouse lung fibroblasts (NFs) and mouse lung CAFs to seek for differentially-expressed secreted proteins. Gene expression microarrays were used to identify transcriptomic changes between NFs and CAFs that may contribute to their different tumor-enhancing capacity. NFs and CAFs were grown in vitro for RNA extraction and hybridization on mouse 430_2 Affymetrix microarrays

2013-07-10 | E-GEOD-48397 | biostudies-arrayexpress

Expression data from human normal fibroblasts (NFs) and cancer associated fibroblasts (CAFs) [HTA-2_0]

Project description:Expression data from human normal fibroblasts (NFs) and cancer associated fibroblasts (CAFs) [HTA-2_0]

| PRJNA630581 | ENA

RNA-Sequencing data of patient derived normal fibroblasts (NFs), cancer associated fibroblasts (CAFs) and tumor spheroid samples

Project description:RNA-Sequencing data of patient derived normal fibroblasts (NFs), cancer associated fibroblasts (CAFs) and tumor spheroid samples performed in the context of the characterization of an IL1R1 positive CAF population in CRC tumour samples.

| EGAS00001007205 | EGA

Gene expression profiles of oral normal fibroblasts (NFs) and oral carcinoma-associated fibroblasts (CAFs)

Project description:This study aims to identify genes differentially expressed in oral CAFs with respect to their normal counterparts, i.e. oral NFs. We collected oral normal mucosal tissues and oral squamous cell carcinoma (OSCC) tissues from 12 healthy people and 12 OSCC patients, respectively, at West China Hospital of Stomatology, Sichuang University, P. R. China. The tissues were isolated, cultured and purified using standard protocols to obtain oral NFs and CAFs. Agilent 4×44K Whole Genome Oligo Microarrays were used to measure the gene expression profiles of the samples. Sample preparation and hybrid reactions were performed according to the manufacture’s instructions. RNA samples from NFs and CAFs were pooled respectively before hybridization. The chips were scanned and visualized using the Agilent DNA microarray scanner. Probe set intensities were measured using Agilent Feature Extraction software (version 10.5.1.1). Raw data were normalized in the Agilent GeneSpring GX software (version 11.0) using the Agilent FE one-color scenario (mainly median normalization). Oral NFs and CAFs were obtained from tissues derived from 12 healthy people undergoing plastic surgery and 12 OSCC patiens undergoing surgical resection. RNA samples from NFs and CAFs were pooled respectively before hybridization.

2012-01-26 | E-GEOD-35356 | biostudies-arrayexpress

Expression data from (mouse) normal lung fibroblasts and carcinoma-associated fibroblasts

2013-07-10 | GSE48397 | GEO

RNA Expression Profile of normal fibroblasts (NFs) and cancer-associated fibroblasts (CAFs) derived from human Pancreatic Ductal Adenocarcinoma.

Project description:Although how CAFs impact the tumor epithelium is being progressively unveiled, transcriptional processes of lineage plasticity in fibroblasts govern the acquisition and transition of the CAF phenotype are much less understood. Here, to explore the potential involvement of the circular RNAs (circRNAs) in fibroblast activation and phenotype acquisition, we isolated CAFs and NFs from human pancreatic cancer and paired normal pancreatic tissue. Next, we conducted circRNA profiling five CAFs and three paired NFs by high throughput sequencing. Our results showed that clusters of circRNAs were aberrantly expressed in CAFs compared with NFs, and provided potential targets for future treatment of PDAC and novel insights into PDAC microenvironment.

2021-04-15 | GSE172096 | GEO

MiRNA expression analysis of cancer-associated fibroblasts (CAFs) and normal fibroblasts (NFs) in breast cancer

Project description:To identify miRNA differentially expressed in CAFs vs matched NFs and analyze the heterogeneity of miRNA expression profiles in the two kinds of cells, we established primary cultures of CAFs and paired NFs from six resected breast tumor tissue without any radiotherapy and chemotherapy treatment. Paired CAFs and NFs from six primary human breast carcinoma specimens were cultured,the third passage of primary cells was used in the experiments.

2012-09-28 | E-GEOD-37527 | biostudies-arrayexpress

Expression data from human normal fibroblasts (NFs) and cancer associated fibroblasts (CAFs)

Project description:CAFs are the major regulators in tumor microenviroment which promote cancer progression. We want to know which miRNAs changed in human CAFs compared to NFs. We used microarrays to detail the mature miRNA expression and identified distinct classes of 2-fold changed miRNAs during this process.

2022-03-28 | GSE128972 | GEO

Gene expression profiles of oral normal fibroblasts (NFs) and oral carcinoma-associated fibroblasts (CAFs)

2012-01-26 | GSE35356 | GEO