Cloeon dipterum
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA530808 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| CATIVA01.dat.gz | Other | |||
| CATIVA01.fasta.gz | Fasta.gz | |||
| CATIVA01.master.dat | Other | |||
| CATIVH01.dat.gz | Other |
Items per page: 5 1 - 5 of 28 |
Similar Datasets
Project description:Cloeon dipterum strain:GR | isolate:male_nymph Raw sequence reads
| PRJNA515246 | ENA
Project description:Testing for toxicity in a number of aquatic organisms is necessary for risk assessments of substances. Yet, aquatic larvae of the so-called EPT (Ephemeroptera, Plecoptera, Trichoptera) taxa are regularly exposed to several environmental contaminants and have been demonstrated to be extremely vulnerable to a variety of environmental pollutants. These results show that existing toxicity testing can result in an underestimating of the risk for EPT taxa and that more toxicity data using EPT taxonomic representatives are needed. Unfortunately, there is a dearth of standardized test techniques and scant published data, particularly for European EPT species. Our study's objective was to create a testing framework for a variety of endpoints in the mayfly Cloeon dipterum. Due to its high prevalence in local waterbodies and its brief lifecycle of a few weeks in the right environmental conditions, C. dipterum was selected as the test organism. To this end, two chronic toxicity tests with semi-static test design and two media renewal per week were performed. Small larvae in the stage L3 based on the wing pad development described by Cianciara (1976) were used in the tests. Four replicates per test concentration and control containing five individuals per replicate were installed. The emergence was determined daily on working days. The tests were conducted at a temperature of 20 °C (± 1 °C) and the illumination was < 1 µE m2s 1 with a light and dark rhythm of 16:8 h. The physico-chemical parameters pH, oxygen concentration, and oxygen saturation were measured using the multiparameter device WTW Multi 1970i at test start, throughout each media renewal, and at test end. Continuous temperature measurements were taken, and weekly lighting assessments were made. Fipronil was administered to C. dipterum larvae in the long-term exposure experiment at nominal concentrations ranging from 0.038 to 0.60 g/L. This test was conducted for 38 days until all larvae had emerged. In the short-term exposure experiment, C. dipterum larvae were exposed to Fipronil in nominal concentrations between 0.038 and 0.30 µg/L for a test duration of seven days. At test end, the larvae were sampled for transcriptome analysis.
2023-11-09 | E-MTAB-12814 | biostudies-arrayexpress
Project description:mRNA-Seq of mayfly (Cloeon dipterum) exposed to different Fipronil concentrations below acute toxicity levels against untreated controls
| PRJEB61040 | ENA
Project description:we used next-generation sequencing technology to characterize mRNA-seq of Cloeon viridulum (Ephemeroptera: Baetidae) among the young larva (YL), mature larva (ML), subimago (SI), imago (IM) stages, and mixture sampling (composite group, CG).
2018-06-01 | GSE101395 | GEO
Project description:In the Paleozoic era, more than 400 Ma, a number of insect groups continued molting after forming functional wings. Today, however, flying insects stop molting after metamorphosis when they become fully winged. The only exception is the mayflies (Paleoptera, Ephemeroptera), which molt in the subimago, a flying stage between the nymph and the adult. However, the identity and homology of the subimago still is underexplored. Debate remains regarding whether this stage represents a modified nymph, an adult, or a pupa like that of butterflies. Another relevant question is why mayflies have the subimago stage despite the risk of molting fragile membranous wings. These questions have intrigued numerous authors, but nonetheless, clear answers have not yet been found. By combining morphological studies, hormonal treatments, and molecular analysis in the mayfly Cloeon dipterum, we found answers to these old questions. We observed that treatment with a juvenile hormone analog in the last nymphal instar stimulated the expression of the Kr-h1 gene and reduced that of E93, which suppress and trigger metamorphosis, respectively. The regulation of metamorphosis thus follows the MEKRE93 pathway, as in neopteran insects. Moreover, the treatment prevented the formation of the subimago. These findings suggest that the subimago must be considered an instar of the adult mayfly. We also observed that the forelegs dramatically grow between the last nymphal instar, the subimago, and the adult. This necessary growth spread over the last two stages could explain, at least in part, the adaptive sense of the subimago.
| S-EPMC8403931 | biostudies-literature
Project description:Genomic adaptations to aquatic and aerial life in mayflies and the origin of wings in insects
| PRJEB34721 | ENA
Project description:The great capability of insects to adapt to new environments promoted their extraordinary diversification, resulting in the group of Metazoa with the largest number of species distributed worldwide. To understand this enormous diversity, it is essential to investigate lineages that would allow the reconstruction of the early events in the evolution of insects. However, research on insect ecology, physiology, development and evolution has mostly focused on few well-established model species. The key phylogenetic position of mayflies within Paleoptera as the sister group of the rest of winged insects and life history traits of mayflies make them an essential order to understand insect evolution. Here, we describe the establishment of a continuous culture system of the mayfly Cloeon dipterum and a series of experimental protocols and omics resources that allow the study of its development and its great regenerative capability. Thus, the establishment of Cloeon as an experimental platform paves the way to understand genomic and morphogenetic events that occurred at the origin of winged insects.
| S-EPMC6446309 | biostudies-literature
Project description:De novo transcriptome of the mayfly Cloeon viridulum and transcriptional signatures of Prometabola
| PRJNA394104 | ENA