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MRNA sequencing of msh1 memory line and non-memory (normal looking) sibling plants with isogenic Col-0 wild-type control in Arabidopsis

ABSTRACT: mRNA sequencing of msh1 memory line and non-memory (normal looking) sibling plants with isogenic Col-0 wild-type control in Arabidopsis

PROVIDER: PRJNA530985 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8850910_1.fastq.gz	Fastqsanger.gz
	SRR8850910_2.fastq.gz	Fastqsanger.gz
	SRR8850911_1.fastq.gz	Fastqsanger.gz
	SRR8850911_2.fastq.gz	Fastqsanger.gz
	SRR8850912_1.fastq.gz	Fastqsanger.gz

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Bisulfite (methylome) sequencing of msh1 memory line and non-memory (normal looking) sibling plants with isogenic Col-0 wild-type control in Arabidopsis

Project description:Bisulfite (methylome) sequencing of msh1 memory line and non-memory (normal looking) sibling plants with isogenic Col-0 wild-type control in Arabidopsis

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mRNA sequencing of msh1 memory line and non-memory (normal looking) sibling plants with isogenic Col-0 wild-type control in Arabidopsis

Project description:Col-0 plants were transformed with MSH1 RNAi construct. Transgene positive plant was self pollinated and transgene was segregated in subsequent generation, and screened for presence of transgene using PCR assay. Of transgene null plants, 20% plants displayed delayed in flowering, smaller in size, and lighter green termed as memory phenotype. Memory plants were self pollinated for six generations and plants from generation 1 and 5 were sequenced for RNASeq.

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Bisulfite (methylome) sequencing of msh1 memory line and non-memory (normal looking) sibling plants with isogenic Col-0 wild-type control in Arabidopsis

Project description:Col-0 plants were transformed with MSH1 RNAi construct. Transgene positive plant was self pollinated and transgene was segregated in subsequent generation, and screened for presence of transgene using PCR assay. Of transgene null plants, 20% plants displayed delayed in flowering, smaller in size, and lighter green termed as memory phenotype. While rest transgene null plants displayed normal phenotype termed as non-memory.

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Bisulfite (methylome) sequencing of msh1 memory line and non-memory (normal looking) sibling plants with isogenic Col-0 wild-type control in Arabidopsis [generation 2 to 6]

Project description:Bisulfite (methylome) sequencing of msh1 memory line and non-memory (normal looking) sibling plants with isogenic Col-0 wild-type control in Arabidopsis [generation 2 to 6]

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Project description:Germination of the memory line and isogenic Col-0 wild type on media containing 100 uM 5-azacytidine alleviated the phenotype differences between the two lines, resulting in similar growth rates, here we generate gene expression profile for wild type and msh1 memory line under 100 uM 5-azacytidine treatment to investigate the effect of 5-azacytidine on msh1 memory gene expression profile

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Bisulfite (methylome) sequencing of msh1 memory line and non-memory (normal looking) sibling plants with isogenic Col-0 wild-type control in Arabidopsis [generation 2 to 6]

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Project description:Germination of the memory line and isogenic Col-0 wild type on media containing 100 uM 5-azacytidine alleviated the phenotype differences between the two lines, resulting in similar growth rates. Here we generate methylome profile for wild type and msh1 memory line under 100 uM 5-azacytidine treatment to investigate the effect of 5-azacytidine on msh1 memory line methylation profile.

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2022-07-08 | GSE192432 | GEO

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