Project description:Transcriptome expression analysis in peanut to date has been limited to a relatively small set of genes and only recently have moderately significant number of ESTs has been released into the public domain. Utilization of these ESTs for the oligonucleotide microarrays provides a means to investigate large-scale transcript responses to a variety of developmental and environmental signals, ultimately improving our understanding of plant biology. We have developed a high-density oligonucleotide microarray for peanut using approximately 47,767 publicly available ESTs and tested the utility of this array for expression profiling in a variety of peanut tissues. To identify putatively tissue-specific genes and investigate the utility of this array, we compared transcript levels in pod to peg, leaf, stem, and root tissues. Results from this experiment showed a number of putatively pod-specific/abundant genes, as well as transcripts whose expression was low or undetected in pod compared to either peg, leaf, or stem. Keywords: Peanut tissue-specific gene expression We used Agilent peanut gene chips (017430) to identify putative tissue-specific genes and investigate the utility of the array for expression profiling of various peanut tissues. Pod, leaf, stem, peg and root tissues of the peanut genotype Flavrunner 458 were used in the study. Field grown plants under normal irrigation were used for sample collection. Three replications of microarray experiments were carried out by hybridizing the cRNA from pod tissue and cRNA from leaf, stem, peg and root tissues on the same dual color oligonucleotide arrays.

2008-12-30 | E-GEOD-11365 | biostudies-arrayexpress

peanut rhizosphere microbiome

Project description:peanut rhizosphere microbiome Raw sequence reads

| PRJNA827859 | ENA

peanut rhizosphere bacterial community

Project description:peanut rhizosphere bacterial community

| PRJNA636625 | ENA

Peanut field bacterial and fungal communities in Florida

Project description:Bacterial and fungal communities in root-knot nematode affected peanut field in Florida

| PRJNA644000 | ENA

CC027/GeniUnc mouse strain-derived jejunal epithelial cells before and after peanut sensitization and at 30, 60, and 120 minutes after peanut challenge

Project description:Peanut allergy reaction severity correlates with increased intestinal epithelial cell (IEC) barrier permeability. CC027/GeniUnc mice develop peanut allergy by intragastric administration of peanut proteins without adjuvant. We report that peanut-allergic CC027/GeniUnc mice showed increased IEC barrier permeability and systemic peanut allergen Ara h 2 after challenge. Jejunal epithelial cell transcriptomics showed effects of peanut allergy on IEC proliferation, survival, and metabolism, and revealed IEC-predominant angiopoietin like-4 (Angptl4) as a unique feature of CC027/GeniUnc peanut allergy. Peanut-allergic pediatric patients demonstrated significantly higher serum ANGPTL4 compared to non-peanut-allergic but atopic patients, highlighting its potential as a biomarker of peanut allergy.

2021-07-09 | GSE179595 | GEO

peanut rhizosphere microbial community

Project description:peanut rhizosphere microbial community Genome sequencing

| PRJNA592826 | ENA

peanut rhizosphere microbial community

Project description:peanut rhizosphere microbial community Genome sequencing

| PRJNA575117 | ENA