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Bronchioalveolar stem cells are a main source for regeneration of distal lung epithelia

ABSTRACT: Bronchioalveolar stem cells are a main source for regeneration of distal lung epithelia

PROVIDER: PRJNA531375 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8863812.fastq.gz	Fastqsanger.gz
	SRR8863813.fastq.gz	Fastqsanger.gz
	SRR8863814.fastq.gz	Fastqsanger.gz
	SRR8863815.fastq.gz	Fastqsanger.gz
	SRR8863816.fastq.gz	Fastqsanger.gz

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Bronchioalveolar stem cells are a main source for regeneration of distal lung epithelia

Project description:Bronchioalveolar stem cells (BASCs) are a potential source for lung regeneration, but direct in vivo evidence is critically missing since specific genetic labeling of BASCs has not been possible. We developed a novel cell tracing approach based on intein-mediated assembly of newly engineered split-effectors, allowing selective targeting of dual-marker expressing BASCs. We found that BASCs generate the majority of distal lung airway cells after bronchioalveolar damage but only moderately contribute to cellular turnover under homeostatic conditions. Importantly, DTA-mediated ablation of BASCs compromised proper regeneration of distal airways. The study defines BASCs as crucial components of the lung repair machinery and provides a paradigmatic example for the detection and manipulation of stem cells that cannot be recognized by a single marker alone.

2019-04-24 | GSE129440 | GEO

Bronchioalveolar stem cells are a main source for regeneration of distal lung epithelia

Project description:Bronchioalveolar stem cells (BASCs) are a potential source for lung regeneration, but direct in vivo evidence for a multipotential lineage contribution during homeostasis and disease is critically missing, since specific genetic labeling of BASCs has not been possible. We developed a novel cell tracing approach based on intein-mediated assembly of newly engineered split-effectors, allowing selective targeting of dual-marker expressing BASCs in the mouse lung. RNA sequencing of isolated BASCs demonstrate that BASCs show a distinct transcriptional profile, characterized by co-expression of bronchiolar and alveolar epithelial genes. We found that BASCs generate the majority of distal lung airway cells after bronchioalveolar damage but only moderately contribute to cellular turnover under homeostatic conditions. Importantly, DTA-mediated ablation of BASCs compromised proper regeneration of distal airways. The study defines BASCs as crucial components of the lung repair machinery and provides a paradigmatic example for the detection and manipulation of stem cells that cannot be recognized by a single marker alone.

| S-SCDT-EMBOJ-2019-102099 | biostudies-other

Bronchioalveolar stem cells are a main source for regeneration of distal lung epithelia in vivo.

Project description:Bronchioalveolar stem cells (BASCs) are a potential source for lung regeneration, but direct in vivo evidence for a multipotential lineage contribution during homeostasis and disease is critically missing, since specific genetic labeling of BASCs has not been possible. We developed a novel cell tracing approach based on intein-mediated assembly of newly engineered split-effectors, allowing selective targeting of dual-marker expressing BASCs in the mouse lung. RNA sequencing of isolated BASCs demonstrates that BASCs show a distinct transcriptional profile, characterized by co-expression of bronchiolar and alveolar epithelial genes. We found that BASCs generate the majority of distal lung airway cells after bronchiolar damage but only moderately contribute to cellular turnover under homeostatic conditions. Importantly, DTA-mediated ablation of BASCs compromised proper regeneration of distal airways. The study defines BASCs as crucial components of the lung repair machinery and provides a paradigmatic example for the detection and manipulation of stem cells that cannot be recognized by a single marker alone.

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Pulmonary fibrosis distal airway epithelia are dynamically and structurally dysfunctional

Project description:To identify genes regulating the jamming transition in healthy distal airway epithelia compared to the dysregulated state in idiopathic pulmonary fibrosis epithelia (IPF) we analyzed RNA from three (3) healthy and four (4) IPF patient cells grown at air-liquid-interface (ALI). This bulk analysis of 3 timepointes (days 4, 8, and 14 of ALI) spanning the jamming transition revealed an enrichment for ERBB- and YAP-related genes.

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Transcriptomic analysis of Campylobacter jejuni grown in medium containing serine as a main energy source

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