Project description:OsTMF is a transcription factor and negatively regulates cold tolerance We used microarray analysis to find differentially expressed genes in OsTMF overexpression (OsTMF-OE) plants compared with the wild-type rice ZH11.
Project description:It is important to reveal the regulatory mechanism of OsMYB30 gene which participated in cold response. We used microarrays to find differentially expressed genes that might be regulated by OsMYB30 and resolve its regulation mechanism. The rice gene OsMYB30 was overexpressed in the two OsMYB30 overexpression plants (OE2 and OE28) compared to the control plant (CK, ZH11). The rice gene OsMYB30 was knocked out in the two osmyb30 homozygous mutants (mutant1 and mutant2) compared to the wild type (WT, Huayang).OE2-C0h and OE28-C0h are two repititions of OsMYB30 overexpression plants under normal conditions with CK-C0h used as control plant. OE2-C6h and OE28-C6h are two repititions of OsMYB30 overexpression plants under cold stress conditions with CK-C6h used as control plant. mutant1-C0h and mutant2-C0h are two repititions of osmyb30 homozygous mutant plants under normal conditions with WT-C0h used as control plant. mutant1-C6h and mutant2-C6h are two repititions of osmyb30 homozygous mutant plants under cold stress conditions with WT-C6h used as control plant.
Project description:St (common potato) is a freezing sensitive species unable to cold acclimate. The close wild relative Sc is freezing tolerant and able to cold acclimate. Here we compare the cold transcriptome of these two species with different levels of freezing tolerance. We also identify the putative CBF regulons by comparing the transcriptomes of wild type plants with that of 35S::AtCBF3 transgenic lines in both species. Plants were grown in 16:8 photoperiod. Eight hours after dawn, plants were either transfered to cold or kept in the warn. Wild type S. tuberosum and S. commersonii were grown at 2oC for 2h, 24h and 7 days. Wild type plants grown under warm temperatures for 2h was used as control for 2h cold samples; wild type warm grown plants for 24h were used as controls for 24h and 7 days cold samples. Under warm conditions, S. commersonii 35S::AtCBF3 lines were compared to S. commersonii wild type plants (same thing was done for S. tuberosum).
Project description:St (common potato) is a freezing sensitive species unable to cold acclimate. The close wild relative Sc is freezing tolerant and able to cold acclimate. Here we compare the cold transcriptome of these two species with different levels of freezing tolerance. We also identify the putative CBF regulons by comparing the transcriptomes of wild type plants with that of 35S::AtCBF3 transgenic lines in both species.
Project description:To identify genes involved in the OsDIS1-mediated drought-responsive pathway, we performed microarray analysis of the OsDIS1 overexpression and wild-type plants under both normal and drought stress conditions using the Agilent rice Genechip. Seven-day-old plants of the OsDIS1 overexpression line 9-4-2 as well as the wild-type plants were used in the drought treatment. OsLEA3 was used as a positive control for the drought treatment. Genes with more than two-fold changes in the overexpression plants compared with the wild-type plants were selected. The expression pattern of some differentially expressed genes was further confirmed by real-time PCR.
Project description:Temperature reduction is a common environmental stress for plants. Land plants need to cope with cold stress on the basis of complex transcriptional and metabolic changes. The transcriptional responses and signaling networks that contribute to cold acclimation of seed plants have been analyzed previously. Here, we present the whole-genome transcriptomic cold stress response of the model moss species Physcomitrella patens as the representative of an early diverged lineage of haploid-dominant and poikilohydric land plants On the basis of time-series microarray experiments we characterized transcriptomic changes related to early stress signaling and the initiation of cold-acclimatory mechanisms, and as secondary effects, of dehydration and oxidative stress.
Project description:RNA-Seq was performed to study the change of gene expression before and after cold treatment in Brachypodium. Different change patterns were identified. We have provided a complete view of transcriptome under cold stress condition, which will deepen our understanding of gene expression regulation in cold stress response as well as cold stress response mechanism for monocot plants.
Project description:Jojoba (Simmondsia chinensis) is a new semi- arid, oil- producing industrial crop that has attracted much attention in recent years. Low temperature is one of the major environmental stress that impairs plant growth and development. To better understand the molecular mechanisms of cold stress adaptation and acclimation of jojoba plants, a quantitative proteomic analysis using iTRAQ technology was conducted to detect the effects of cold stress on protein expression profiles in jojoba seedlings. Our work provided useful infomation for understanding the cold stress response and cold acclimation in jojoba.
Project description:To identify the target genes regulated by ANAC069, the expression profiles of OE (Overexpression of ANAC069) plants and KO (Knockout of ANAC069, SALK_095231C) plants under salt stress conditions were compared using Affymetrix Arabidopsis gene chips.