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Paeonia suffruticosa

ABSTRACT: Transcriptomic study of floral development in tree peony

PROVIDER: PRJNA531582 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8903930_1.fastq.gz	Fastqsanger.gz
	SRR8903930_2.fastq.gz	Fastqsanger.gz
	SRR8903931_1.fastq.gz	Fastqsanger.gz
	SRR8903931_2.fastq.gz	Fastqsanger.gz
	SRR8903928_subreads.fastq.gz	Fastqsanger.gz

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Paeonia suffruticosa

Project description:tree peony, native to China

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Fatty acid composition of developing tree peony (Paeonia section Moutan DC.) seeds and transcriptome analysis during seed development

Project description:Tree peony (Paeonia ostii section Moutan DC.) is known for its excellent ornamental and medicinal values. In 2011, seeds from P. ostii have been identified as novel resource of alpha-linolenic acid (ALA) for seed oil production and development in China. However, the molecular mechanism on biosynthesis of unsaturated fatty acids in tree peony seeds remains unknown. Therefore, transcriptome data is needed to better understand the underlying mechanisms. In this study, lipids accumulation contents were measured using GC-MS methods across developing tree peony seeds, which exhibited an extraordinary ALA content (49.3%) in P. ostii mature seeds. Transcriptome analysis was performed using Illumina sequencing platform. A total of 144 million 100-bp paired-end reads were generated from six libraries, which identified 175,874 contigs. In the KEGG Orthology enrichment of differentially expressed genes, lipid metabolism pathways were highly represented categories. Using this data we identified 388 unigenes that may be involved in de novo fatty acid and triacylglycerol biosynthesis. In particular, three unigenes (SAD, FAD2 and FAD8) encoding fatty acid desaturase with high expression levels in the fast oil accumulation stage compared with the initial stage of seed development were identified.

2017-03-04 | E-MTAB-3377 | biostudies-arrayexpress

Paeonia suffruticosa breed:Okan | cultivar:Okan

Project description:Tree peony radiationed Raw sequence reads

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Transcriptome profiling for floral bud development in reblooming cultivar 'High Noon' of Paeonia suffruticosa

Project description:To examine the mechanisms that control flower development, we sequenced the flower bud transcriptomes of ‘High Noon’, a reblooming cultivar of P. suffruticosa × P. lutea. Both full-length isoforms and RNA-seq were sequenced in 3 floral developmental stages. A total of 15.94 Gb raw data and 457.0 million reads were generated in full-length transcript sequencing and RNA-seq.

2019-06-29 | GSE133476 | GEO

Transcript profiling in Paoenia ostii during chilling induced endo-dormancy release

Project description:To better understanding the genetic and physiological changes behind the dormancy process in tree peony, we performed customized cDNA microarray to investigate gene expression profiling in tree peony ‘Feng Dan Bai’ buds during chilling induced dormancy release. Endo-dormant tree peony plants were exposed to 0-4°C from 5 November to 30 December 2009 in Qingdao, Shandong, China. Buds were collected after 0 d, 6 d, 12 d, 15 d, 18 d and 24 d chilling endured. DNA microarrays were customized using Agilent eArray 5.0 program, containing spots with 14,957 gene-specific 60-mer oligonucleotides representing 14,957 non abundant ESTs obtained from 454 sequencing normalized cDNA of tree peony buds during chilling duration (TSA, 65,217). Total 3,174 significantly differentially-expressed genes (P<0.05) were observed through endo-dormancy release, and the number of up-regulated (1,611) and that of down-regulated (1,563) was almost same. Expression of differentially-expressed genes associated with GA biosynthesis and signaling, cell growth and development was confirmed by quantitative RT-PCR, which displayed similar trends pattern in expression.

2014-08-01 | GSE40004 | GEO

Transcript profiling in Paoenia ostii during chilling induced endo-dormancy release

Project description:To better understanding the genetic and physiological changes behind the dormancy process in tree peony, we performed customized cDNA microarray to investigate gene expression profiling in tree peony M-bM-^@M-^XFeng Dan BaiM-bM-^@M-^Y buds during chilling induced dormancy release. Endo-dormant tree peony plants were exposed to 0-4M-BM-0C from 5 November to 30 December 2009 in Qingdao, Shandong, China. Buds were collected after 0 d, 6 d, 12 d, 15 d, 18 d and 24 d chilling endured. DNA microarrays were customized using Agilent eArray 5.0 program, containing spots with 14,957 gene-specific 60-mer oligonucleotides representing 14,957 non abundant ESTs obtained from 454 sequencing normalized cDNA of tree peony buds during chilling duration (TSA, 65,217). Total 3,174 significantly differentially-expressed genes (P<0.05) were observed through endo-dormancy release, and the number of up-regulated (1,611) and that of down-regulated (1,563) was almost same. Expression of differentially-expressed genes associated with GA biosynthesis and signaling, cell growth and development was confirmed by quantitative RT-PCR, which displayed similar trends pattern in expression. Transcript profiling of tree peony was measured during chilling (0-4M-BM-0C) induced dormancy release. Mixed buds, three buds for each individual, were collected after 0, 6, 12, 15, 18 (endo-dormancy release), 24 days (eco-dormancy) chilling requirement fulfilling. Three replications (3 plants/ replication) were harvested between November and December.

2014-08-01 | E-GEOD-40004 | biostudies-arrayexpress

Identification of genes associated with biosynthesis of unsaturated fatty acid and oil accumulation in herbaceous peony 'Hangshao' (Paeonia lactiflora 'Hangshao') seeds based on transcriptome analysis

Project description:High-throughput RNA sequencing were performed to identify a large number of mRNAs in developmental herbaceous peony seeds at different periods to provide useful information for elucidating the molecular mechanism of the lipid biosynthesis and fatty acid accumulation for herbaceous peony 'Hangshao'.

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Project description:Paeonia suffruticosa Raw sequence reads

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Project description:Paeonia suffruticosa

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Project description:Paeonia suffruticosa Transcriptome or Gene expression

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