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Florigen family chromatin recruitment, competition and targets [ChIP-Seq]

ABSTRACT: Florigen family chromatin recruitment, competition and targets [ChIP-Seq]

PROVIDER: PRJNA531668 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10676619.fastq.gz	Fastqsanger.gz
	SRR10676620.fastq.gz	Fastqsanger.gz
	SRR10676621.fastq.gz	Fastqsanger.gz
	SRR10676622.fastq.gz	Fastqsanger.gz
	SRR10676623.fastq.gz	Fastqsanger.gz

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Florigen family chromatin recruitment, competition and targets

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Florigen family chromatin recruitment, competition and targets [ChIP-Seq]

Project description:This study identified direct targets of TFL1 and FD and showed that TFL1 is recruited to the chromatin by FD. We performed ChIP-seq using anti-GFP antibodies in gTFL1-GFP tfl1-1, gTFL1-GFP fd-1 and controls (nontransgenic plants). We used anti-GUS antibodies for gFD-GUS fd-1. ChIP was conducted on dissected, non-bolted, inflorescences from 42-day-old short-day-grown Arabidopsis thaliana.

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Florigen family chromatin recruitment, competition and targets

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Florigen family chromatin recruitment, competition and targets [RNA-Seq]

Project description:This study identified genes that re-upregulated in far-red enriched photoperiod in the wild type or tfl1-1, but not in ft-10 (FT-dependent photoinduction). We performed RNA seq on tfl1-1, wild type and ft-10 grown for 42 days in short-day conditions and treated for 24 hours with far-red enriched photoinduction (24 hr FRP) or grown for 43 days in short-day conditions (no FRP). RNA was isolated from trimmed and dissected non-bolted inflorescences.

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