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M6A modification in effector CD4+ T cells [m6A-miCLIP-seq]

ABSTRACT: m6A modification in effector CD4+ T cells [m6A-miCLIP-seq]

PROVIDER: PRJNA532394 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12162935_1.fastq.gz	Fastqsanger.gz
	SRR12162935_2.fastq.gz	Fastqsanger.gz
	SRR12162936_1.fastq.gz	Fastqsanger.gz
	SRR12162936_2.fastq.gz	Fastqsanger.gz
	SRR12162937_1.fastq.gz	Fastqsanger.gz

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m6A modification in effector CD4+ T cells [m6A-miCLIP-seq]

Project description:GP61-primed effector CD4+ T cells were isolated from Ctrl or Mettl3-deficient SMARTA mice. Total RNAs were extracted with TRIzol reagent, and mRNAs were then isolated with Dynabeads® mRNA purification kit, followed by stardard m6A-miCLIP-SMARTer-seq with some modifications. Raw sequencing reads were aligned to the mouse genome (mm10) with BWA, and then m6A sites were determined.

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Mettl3-dependent m6A modification is essential for effector differentiation and memory formation of CD8+ T cells [miCLIP]

Project description:The goal of this study is to determine the critical requirement of N6-Methyladenosine (m6A) methyltransferase Mettl3 during CD8+ T cell responses upon acute viral infection. We demonstrate that conditional ablation of Mettl3 in CD8+ T cells impairs effector expansion and terminal differentiation in an m6A-dependent manner, which in turn affects memory formation and secondary response. Mechanism analysis indicates that Mettl3 deficiency broadly affects expression of cell cycle and transcriptional regulators. Based on these findings, our study reveals the important role of m6A modification in CD8+ T cell responses.

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