Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Long non-coding RNA GRASLND enhances chondrogenesis via suppression of interferon type II signaling pathway

ABSTRACT: Long non-coding RNA GRASLND enhances chondrogenesis via suppression of interferon type II signaling pathway

PROVIDER: PRJNA533337 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8924620_1.fastq.gz	Fastqsanger.gz
	SRR8924620_2.fastq.gz	Fastqsanger.gz
	SRR8924617_1.fastq.gz	Fastqsanger.gz
	SRR8924617_2.fastq.gz	Fastqsanger.gz
	SRR8924618_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Long non-coding RNA GRASLND enhances chondrogenesis via suppression of interferon type II signaling pathway

Project description:Long non-coding RNAs (lncRNAs) play critical roles in regulating gene expression and cellular processes; however, their roles in musculoskeletal development, disease, and regeneration remain poorly understood. Here, we identified a novel lncRNA, Glycosaminoglycan Regulatory ASsociated Long Non-coDing RNA (GRASLND), as a regulator of mesenchymal stem cell (MSC) chondrogenesis, and we investigated its basic molecular mechanism and its potential application towards regenerative medicine. GRASLND, a primate-specific lncRNA, is upregulated during MSC chondrogenesis and appears to act directly downstream of SRY-Box 9 (SOX9), but not Transforming Growth Factor Beta 3 (TGF-β3). Utilizing the established model of pellet formation for MSC chondrogenesis, we showed that the silencing of GRASLNDresulted in lower accumulation of cartilage-like extracellular matrix, while GRASLND overexpression, either via transgene ectopic expression or by endogenous activation via CRISPR, significantly enhanced cartilage matrix production. GRASLND acts to inhibit IFN-γ by binding to eukaryotic initiation factor-2 kinase PKR. We further demonstrated that GRASLND exhibited a protective effect in engineered cartilage against interferon type II, and that GRASLND exerted this effect similarly in engineered cartilage across different sources of progenitors. Our results indicate an important role of GRASLND in regulating stem cell chondrogenesis, as well as its therapeutic potential in the treatment of cartilage-related diseases, such as osteoarthritis.

2020-04-06 | GSE129985 | GEO

Long non-coding RNA GRASLND enhances chondrogenesis via suppression of the interferon type II signaling pathway.

Project description:The roles of long noncoding RNAs (lncRNAs) in musculoskeletal development, disease, and regeneration remain poorly understood. Here, we identified the novel lncRNA GRASLND (originally named RNF144A-AS1) as a regulator of mesenchymal stem cell (MSC) chondrogenesis. GRASLND, a primate-specific lncRNA, is upregulated during MSC chondrogenesis and appears to act directly downstream of SOX9, but not TGF-β3. We showed that the silencing of GRASLND resulted in lower accumulation of cartilage-like extracellular matrix in a pellet assay, while GRASLND overexpression - either via transgene ectopic expression or by endogenous activation via CRISPR-dCas9-VP64 - significantly enhanced cartilage matrix production. GRASLND acts to inhibit IFN-γ by binding to EIF2AK2, and we further demonstrated that GRASLND exhibits a protective effect in engineered cartilage against interferon type II. Our results indicate an important role of GRASLND in regulating stem cell chondrogenesis, as well as its therapeutic potential in the treatment of cartilage-related diseases, such as osteoarthritis.

| S-EPMC7202894 | biostudies-literature

Suppression of Ca2+ Signaling Enhances Melanoma Progression

Project description:Suppression of Ca2+ Signaling Enhances Melanoma Progression

| PRJNA847914 | ENA

Suppression of Progenitor Differentiation Requires the Long Non-Coding RNA ANCR

Project description:This SuperSeries is composed of the following subset Series: GSE34528: Suppression of Progenitor Differentiation Requires the Long Non-Coding RNA ANCR [HG-U133_Plus_2] GSE34766: Suppression of Progenitor Differentiation Requires the Long Non-Coding RNA ANCR [lincRNA 391k Tiling Array V2] Refer to individual Series

2012-01-01 | E-GEOD-34767 | biostudies-arrayexpress

Chondrogenesis in ATDC5 cells

Project description:SAGE analysis of early chondrogenesis in ATDC5 cells induced by BMP4 Keywords: time-course

2003-09-10 | E-GEOD-652 | biostudies-arrayexpress

Chondrogenesis in ATDC5 cells

Project description:SAGE analysis of early chondrogenesis in ATDC5 cells induced by BMP4 Keywords: time-course

2003-09-10 | GSE652 | GEO

Angiotensin II-induced long non-coding RNA Alivec regulates chondrogenesis in vascular smooth muscle cells

Project description:Angiotensin II-induced long non-coding RNA Alivec regulates chondrogenesis in vascular smooth muscle cells

| PRJNA762117 | ENA

Human bone-marrow-derived mesenchymal stem cells: control vs. cells undergoing chondrogenesis

Project description:Long non-coding RNAs profiling of human mesenchymal stem cells comparing undifferentiated HMSCs with differentiated HMSCs during chondrogenesis. The chondrogenic differentiation of HMSCs was induced by chondrogenic medium. The chondrogenic marker genes (Col2a1, Sox9 and ACAN) has been detected upregulating during this process by Q-PCR. The aim of this study is to determine key lncRNAs regulating the chondrogenic differentiation process.

2020-03-18 | GSE128533 | GEO

Suppression of Progenitor Differentiation Requires the Long Non-Coding RNA ANCR

Project description:Suppression of Progenitor Differentiation Requires the Long Non-Coding RNA ANCR

| PRJNA150347 | ENA

Suppression of Ca2+ Signaling Enhances Melanoma Progression

Project description:To investigate the effect of UV on SOCE suppression in melanoma progression We performed gene expression analysis of cells exhibiting SOCE suppression and cells not exhibiting SOCE suppression compared to control

2022-06-17 | GSE205830 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data