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Analysis of the expression profile of skin fibroblasts FACS-sorted from mice overexpressing activin and/or oncogenes of human papilloma virus 8 in keratinocytes.

ABSTRACT: Analysis of the expression profile of skin fibroblasts FACS-sorted from mice overexpressing activin and/or oncogenes of human papilloma virus 8 in keratinocytes.

PROVIDER: PRJNA533599 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8927050.fastq.gz	Fastqsanger.gz
	SRR8927060.fastq.gz	Fastqsanger.gz
	SRR8927051.fastq.gz	Fastqsanger.gz
	SRR8927052.fastq.gz	Fastqsanger.gz
	SRR8927053.fastq.gz	Fastqsanger.gz

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Analysis of the expression profile of skin macrophages FACS-sorted from mice overexpressing activin and/or oncogenes of human papilloma virus 8 in keratinocytes.

Project description:We have shown that activin promoted skin tumorigenesis in mice induced by the human papilloma virus 8 oncogenes. Activin attracted blood monocytes to the skin as revealed by depletion of CCR2-positive monocytes. To determine if activin also altered the gene expression profile of these cells, we performed RNA-Sequencing of macrophages FACS-sorted from the pre-cancerous ear skin. We have found that activin induces a pro-migratiory, pro-angiogenic and pro-tumorigenic genes in skin macrophages in vivo. This largely contributes to the pro-tumorigenic function of activin, since macrophage depletion delayed spontaneous tumorigenesis in HPV8-transgenic mice by reducing keratinocyte proliferation and angiogenesis.

2017-02-08 | GSE79086 | GEO

Analysis of the expression profile of skin fibroblasts FACS-sorted from mice overexpressing activin and/or oncogenes of human papilloma virus 8 in keratinocytes.

Project description:Overexpression of activin by keratinocytes promotes HPV8-induced skin tumorigenesis in mice. Activin-promoted tumorigenesis is mediated via the stroma, specifically by cancer-associated fibroblasts (CAFs). To determine if activin alters the gene expression profile of skin fibroblasts, we performed RNA-sequencing of fibroblasts FACS-sorted from pre-cancerous ear skin of activin overexpressing mice. We found that activin induces a CAF-like gene expression profile of fibroblasts, leading to the upregulation of genes involved in proliferation, migration and cytoskeletal remodeling.

2020-03-01 | GSE130017 | GEO

Analysis of the expression profiles of back skin and excisional wound-derived fibroblasts FACS-sorted from mice overexpressing activin A.

Project description:Overexpression of activin A by keratinocytes accelerates excisional wound healing in mice. Activin-promoted wound healing is mediated via the stroma, specifically by the wound immune cells and fibroblasts. To determine if activin A alters the gene expression profile of wound fibroblasts, we performed RNA-sequencing of fibroblasts FACS-sorted from excisional skin wounds of activin overexpressing mice. We found that activin induces a pro-fibrotic gene expression profile of these fibroblasts, leading to the upregulation of genes involved in collagen biosynthesis and remodeling.

2020-04-14 | GSE134789 | GEO

Analysis of the expression profiles of back skin and spontaneous back skin tumor-derived fibroblasts FACS-sorted from mice overexpressing oncogenes of human papilloma virus 8 in keratinocytes.

Project description:Analysis of the expression profiles of back skin and spontaneous back skin tumor-derived fibroblasts FACS-sorted from mice overexpressing oncogenes of human papilloma virus 8 in keratinocytes.

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Analysis of the expression profiles of back skin and spontaneous back skin tumor-derived fibroblasts FACS-sorted from mice overexpressing oncogenes of human papilloma virus 8 in keratinocytes.

Project description:Overexpression of the oncogene HPV8 in keratinocytes of mice causes spontaneous papillomas to form in the skin. To study the expression profiles of skin cancer-associated fibroblasts, we performed RNA-sequencing of fibroblasts FACS-sorted from back skin tumors of HPV8 overexpressing mice.

2023-01-01 | GSE182880 | GEO

Transcription profiling of mouse activin treated MEKK1 wild type and KO keratinocytes

Project description:MEKK1 wildtype and knockout keratinocytes were compared both with and without activin (10 ng/ml) treatment for 12 hours, and control vs activin treated cells were compared both for wildtype and knockout. This was performed in a square design.

2007-11-20 | E-MEXP-355 | biostudies-arrayexpress

Activin A regulates human T follicular helper (Tfh) cell differentiation

Project description:To determine the role of the cytokine activin A in the regulation of human T follicular helper (Tfh) cell gene program, we performed a transcriptomic analysis (RNA-seq) of human naÃ¯ve CD4 T cells differentiated in vitro with activin A. The analysis of the gene expression profile driven by activin A, alone or in combination with IL-12 (a know regulator of human Tfh differentiation/function), revealed that activin A can regulate the expression of multiple molecules involved in the differentiation and/or function of human Tfh cells. Human naÃ¯ve CD4 T cells were isolated from fresh PBMCs of healthy control subjects by magnetic bead isolation. Purity was measured by FACS as percentage of CD4+CD45RA+ cells and was 95% or higher. Upon isolation, naÃ¯ve CD4 T cells were stimulated with anti-CD3/CD28 coated beads in the presence of the following cytokine combinations: no exogenous cytokines (beads only), activin A, IL-12, activin A+IL-12, TGFb, TGFb +IL12. Following 5 days of in vitro culture, live CD4 T cells were FACS sorted and gene expression was analyzed by RNA-seq. Data are from independent donors.

2016-06-01 | E-GEOD-78276 | biostudies-arrayexpress

Analysis of the expression profiles of back skin and excisional wound-derived fibroblasts FACS-sorted from mice overexpressing activin A.

Project description:Analysis of the expression profiles of back skin and excisional wound-derived fibroblasts FACS-sorted from mice overexpressing activin A.

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activin A treated Th17 cells from mouse

Project description:Whole cell extracts from mouse Th17 cells treated with activin A were profiled with extensive fractionation and bottom-up proteomics

2020-05-18 | PXD017757 | Pride

Gene expression profiling of Nodal versus Activin-derived endoderm from mouse ES cells

Project description:To explore the molecular basis of functional differences observed between Nodal versus Activin-derived endoderm, we compared their respective gene expression profiles. Sox17-GFP mouse ES cells were differentiated in the presence of Nodal or Activin for 7 days, after which GFP(+) cells were purified by FACs. Undifferentiated ES cells were also included for comparison as a control. Results indicate that the two endoderm populations are nearly identical at the level of global transcription. Subtle differences suggest a difference in the degree of endoderm progression.

2013-01-01 | GSE41086 | GEO

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