Project description:Bone morphogenetic protein-4 (BMP4) is involved in regulation of neural stem cells (NSCs) proliferation, differentiation, migration and survival. It was previously thought that the treatment of NSCs with BMP4 alone induces astrocytes, whereas the treatment of NSCs with the bFGF/BMP4 combination induces quiescent neural stem cells (qNSCs). In this study, we performed RNA sequencing (RNA-Seq) to compare the transcriptome profiles of BMP4-treated NSCs and bFGF/BMP4-treated NSCs, and found that both NSCs treated by these two methods were Sox2 positive qNSCs which were able to generate neurospheres. However, NSCs treated by those two methods exhibited different characteristics in state and the potential for neuronal differentiation based on transcriptome analysis and experimental results. We found that BMP4-treated NSCs tended to be in a deeper quiescent state than bFGF/BMP4-treated NSCs as the percentage of ki67-positive cells were lower in BMP4-treated NSCs. And after exposure to differentiated environment, bFGF/BMP4-treated NSCs generated more DCX-positive immature neurons and MAP2-positive neurons than BMP4-treated NSCs. Our study characterized qNSCs treated with BMP4 alone and bFGF/BMP4 combination, which laid a foundation for studying the activation mechanism of qNSCs.

2024-06-26 | GSE267115 | GEO

Transcriptomic differences between deeply quiescent and activated adult NSCs

Project description:To identify pathways regulating NSC quiescence, with a possible link to quiescence depth, we used double transgenic Tg(her4:drfp);Tg(mcm5:gfp) fish and paradigms predicted to highlight deep vs shallower quiescence. In adult fish (3 month-post-fertilization -mpf-), we performed bulk RNA sequencing on FACS sorted RFPhigh,GFPneg qNSCs (expressing strongly her4, signing NSCs transcriptionally remote from activation), and activated NSCs.

2024-02-20 | GSE239594 | GEO

Gene expression data from mouse ES cell-derived primitive NSCs and definitive NSCs

Project description:Primitive neural stem cells (NSCs) could be derived from pluripotent mouse embryonic stem (ES) cells, and then differentiate into definitive-type neural stem cells which resemble NSCs obtained from the central nervous system. Hence, primitive NSCs define an early stage of neural induction and provide a model to understand the mechanism that controls initial neural commitment. In this study, we performed microarray assay to analyze the global transcriptional profiles in mouse ES cell-derived primitive and definitive NSCs and to depict the molecular changes during the multi-staged neural differentiation process. Primitive NSCs derived directly from ESCs in Lif (p-NSC_L), primitive NSCs that were sub-cultured in the presence of Lif and FGF (p-NSC_LF), as well as definitive NSCs derived from primitive NSCs in medium containing FGF and EGF, were collected for RNA extraction and hybridization on Affymetrix microarrays. Mouse ESCs and NSCs obtained from mouse embryonic brain (E11.5) were included for controls. For each cell type, we collected two biological replicate samples for microarray analysis.

2014-03-01 | E-GEOD-44608 | biostudies-arrayexpress

Gene expression profiles of Ecrg4-wt, -hetero and -homo NSCs

Project description:Gene expression profiles of Ecrg4-wt, -hetero and -homo NSCs

| PRJNA494638 | ENA

Gene expression data from mouse ES cell-derived primitive NSCs and definitive NSCs

2014-03-01 | GSE44608 | GEO

Expression data from rat NSCs co-cultured with green fluorescence protein (GFP)-ectodermal mesenchymal stem cells (EMSCs) versus mono-cultured rat NSCs

Project description:Rat NSCs were isolated from developing whole brian of the SD rat embryos at day 13.5. In the NSC/GFP-EMSC co-culture system, the NSCs showed significantly enhanced neuronal differentiation rather than astrocytic commitment, in comparison with the mono-cultured NSCs. In order to reveal the gene expression profiles of NSCs under these two diffferent culture conditions, we used microassrays to examine the global programme of gene expression of NSCs under these two diffferent culture conditions.

2021-01-03 | GSE108752 | GEO

Transcriptomic differences between juvenile and adult quiescent NSCs

Project description:We generated a dataset in which we compared the bulk transcriptome of qNSCs between 1.5 and 3.5 mpf fish, stages between which deeper quiescence is progressively instated in order to find differences between deeply and shallow quiescent NSC

2024-02-20 | GSE239597 | GEO

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