Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Priming mobilization of hair follicle stem cells triggers permanent loss of regeneration after alkylating chemotherapy

ABSTRACT: Priming mobilization of hair follicle stem cells triggers permanent loss of regeneration after alkylating chemotherapy

PROVIDER: PRJNA533647 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8929421_1.fastq.gz	Fastqsanger.gz
	SRR8929421_2.fastq.gz	Fastqsanger.gz
	SRR8929422_1.fastq.gz	Fastqsanger.gz
	SRR8929422_2.fastq.gz	Fastqsanger.gz
	SRR8929423_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Priming mobilization of hair follicle stem cells triggers permanent loss of regeneration after alkylating chemotherapy

Project description:The maintenance of genetic integrity is critical for stem cells to ensure homeostasis and regeneration. Little is known about how adult stem cells respond to irreversible DNA damage, resulting in loss of regeneration in humans. Here, we establish a permanent regeneration loss model using cycling human hair follicles treated with alkylating agents: busulfan followed by cyclophosphamide. We unravel the underlying mechanisms by which hair follicle stem cells (HFSCs) lose their pool. Contrary to immediate destructive changes in rapidly proliferating hair matrix cells, quiescent HFSCs show unexpected massive proliferation after busulfan and then undergo large-scale apoptosis following cyclophosphamide. HFSC proliferation is activated through PI3K/Akt pathway, and depletion is driven by p53/p38-induced cell death. RNA-seq analysis shows that HFSCs experience mitotic catastrophe with G2/M checkpoint activation. Our findings indicate that priming mobilization causes stem cells to lose their resistance to DNA damage, resulting in permanent loss of regeneration after alkylating chemotherapy.

2019-07-09 | GSE130054 | GEO

Priming mobilization of hair follicle stem cells triggers permanent loss of regeneration after alkylating chemotherapy.

Project description:The maintenance of genetic integrity is critical for stem cells to ensure homeostasis and regeneration. Little is known about how adult stem cells respond to irreversible DNA damage, resulting in loss of regeneration in humans. Here, we establish a permanent regeneration loss model using cycling human hair follicles treated with alkylating agents: busulfan followed by cyclophosphamide. We uncover the underlying mechanisms by which hair follicle stem cells (HFSCs) lose their pool. In contrast to immediate destructive changes in rapidly proliferating hair matrix cells, quiescent HFSCs show unexpected massive proliferation after busulfan and then undergo large-scale apoptosis following cyclophosphamide. HFSC proliferation is activated through PI3K/Akt pathway, and depletion is driven by p53/p38-induced cell death. RNA-seq analysis shows that HFSCs experience mitotic catastrophe with G2/M checkpoint activation. Our findings indicate that priming mobilization causes stem cells to lose their resistance to DNA damage, resulting in permanent loss of regeneration after alkylating chemotherapy.

| S-EPMC6711970 | biostudies-literature

Priming

Project description:Priming

| OEX00014296 | NODE

Less invasive diagnostics for cancer using hair follicle

Project description:Primary outcome(s): Comparison of the gene expression profile in hair follicle between cancer patients and healthy volunteer

| 2628246 | ecrin-mdr-crc

G-CSF plus AMD3100 mobilization in adult patients with beta-thalassemia results in effective mobilization of CD34+ cells with primitive signatures

Project description:Successful gene therapy for Î²-thalassemia requires optimal numbers of autologous gene-transduced hematopoietic progenitors stem cells (HPSC) with high repopulating capacity and mobilization is the optimal approach for achieving this goal. We performed a clinical study to investigate safety and efficacy of G-CSF+AMD3100 mobilization in four adult with Î²-thalassemia to reach a cell dose of â¥8x106 CD34+cells/Kg and to characterize the CD34+cell populations mobilized before and after plerixafor administration. The procedure resulted well-tolerated in all patients. Three of the four patients reached the target cell dose or more in single-apheresis collections, even one patient where a significant dose reduction of G-CSF was imposed due to early hyperleukocytosis. A significant increase in the number of circulating CD34+ cells/Âµl (12.1Â± 8.2 fold), and in the frequency of the more primitive CD34+/CD133+/CD38- cells (1.7Â±0.7 fold) was unanimously observed after AMD3100 addiction. Intraindividual comparison of gene expression profile of CD34+ cells mobilized with G-CSF versus G-CSF+ AMD3100 highlighted a different expression pattern of genes involved in the processes of HSC/stroma adhesion, homing, cell motility and transcription regulation. Overall, the safety profile, the yield, and the expression of genes that potentially promote superior engraftment depict G-CSF+AMD3100 mobilized HSC as optimal graft source for Î²-thalassemia gene therapy One experiment performed on purified CD34+ PBPC of the same patient 1 after mobilization with G-CSF + Plerixafor , and after mobilization with G-CSF alone. Universal Human Reference RNA (Stratagene, La Jolla, CA), was used as reference.

2017-04-28 | E-GEOD-70219 | biostudies-arrayexpress

Gene expression analysis of human permanent dental pulp

Project description:Gene expression analysis data of human permanent dental pulp tissues

2016-11-22 | GSE75644 | GEO

Organosulfur mobilization

Project description:Bacterial mobilization of organically bound sulfur in the mycorrhizosphere to improve plant sulfur supply

| PRJEB37537 | ENA

Gene expression analysis of human permanent periodontal ligament tissue

Project description:Gne expression analysis data of human permanent periodontal ligament tissues

2018-12-31 | GSE75695 | GEO

IL-36? Promoted Wound Induced Hair Follicle Neogenesis via Hair Follicle Stem/Progenitor Cell Proliferation.

Project description:Background:Wound-induced hair follicle neogenesis (WIHN) is a phenomenon of hair neogenesis that occurs at the center of a scar when the wound area is sufficiently large. Neogenic hair follicles are separated from the pre-existing follicles at the wound edge by a hairless circular region. This WIHN study provides a unique model for developing treatments for hair loss and deciphering the mechanisms underlying organogenesis in adult mammals. Methods:The skin of a mouse was wounded by excising a 1.5 × 1.5 cm2 square of full-thickness dorsal skin. iTRAQ technology was used to screen proteins differentially expressed between the inner and outer scar areas in a mouse model of WIHN, on post-wounding day 15, to identify the regulators of WIHN. Owing to the overexpression of interleukin-36? (IL-36?) in the de novo hair follicle growth area, the regulating effect of IL-36? overexpression in WIHN was investigated. Hair follicle stem/progenitor cells were counted by flow cytometry while the expression of hair follicle stem/progenitor cell markers (Lgr5, Lgr6, Lrig1, K15, and CD34) and that of Wnt/?-catenin and IL-6/STAT3 pathway intermediaries was detected by qPCR and western blotting. Results:We found that wounding induced IL-36? expression. Incorporation of recombinant murine IL-36? (mrIL-36?) into murine skin wounds resulted in a greater number of regenerated hair follicles (p < 0.005) and a faster healing rate. The expression of hair follicle stem/progenitor cell markers was upregulated in the mrIL-36?-injected site (p < 0.05). Additionally, mrIL-36? upregulated the IL-6/STAT3 pathway intermediaries. Conclusion:IL-36? is upregulated in de novo hair follicle growth areas and can promote wound epithelialization and WIHN.

| S-EPMC7493638 | biostudies-literature

Whole Exome Sequencing of Permanent Neonatal Diabetes Patients

Project description:Whole Exome Sequencing of Permanent Neonatal Diabetes Patients

| EGAS00001000047 | EGA