Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Enrichment of anaerobic fungi and methanogens produce more methane than enrichment of bacteria and methanogen

PROVIDER: PRJNA533952 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8935711_1.fastq.gz	Fastqsanger.gz
	SRR8935711_2.fastq.gz	Fastqsanger.gz
	SRR8935712_1.fastq.gz	Fastqsanger.gz
	SRR8935712_2.fastq.gz	Fastqsanger.gz
	SRR8935713_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Gene expression of M. acetivorans producing ANME-1 Mcr during growth on methane vs. growth on methanol

Project description:Our goal is to convert methane efficiently into liquid fuels that may be more readily transported. Since aerobic oxidation of methane is less efficient, we focused on anaerobic processes to capture methane, which are accomplished by anaerobic methanotrophic archaea (ANME) in consortia. However, no pure culture capable of oxidizing and growing on methane anaerobically has been isolated. In this study, Methanosarcina acetivorans, an archaeal methanogen, was metabolically engineered to take up methane, rather than to generate it. To capture methane, we cloned the DNA coding for the enzyme methyl-coenzyme M reductase (Mcr) from an unculturable archaeal organism from a Black Sea mat into M. acetivorans to effectively run methanogenesis in reverse. The engineered strain produces primarily acetate, and our results demonstrate that pure cultures can grow anaerobically on methane.

2015-12-20 | GSE66445 | GEO

Project description:Enrichment of microflora with fungi and methanogen to produce methane from coal

| PRJNA657731 | ENA

Time series of Methanococcus maripaludis MM901:del_MMP1447, a shift from a H2-excess and P-limiting condition to a H2-limited and P-excess condition in chemostats (growth rate held constant)

Project description:Methanogens catalyze the critical, methane-producing step (called methanogenesis) in the anaerobic decomposition of organic matter and have applications in carbon-neutral fuel production. Here, we present the first predictive model of global gene regulation of methanogenesis in a hydrogenotrophic methanogen, Methanococcus maripaludis. We generated a comprehensive list of genes (protein-coding and non-coding) for M. maripaludis through integrated analysis of the transcriptome structure and a newly constructed Peptide Atlas. The environment and gene-regulatory influence network (EGRIN) model of the strain was constructed from a compendium of transcriptome data that was collected over 100 different steady-state and time course experiments that were performed in chemostats, or batch cultures, under a spectrum of environmental perturbations that modulated methanogenesis. We discovered that at least five regulatory mechanisms act in a combinatorial scheme to inter-coordinate key steps of methanogenesis with different processes such as motility, ATP biosynthesis, and carbon assimilation. Through a combination of genetic and environmental perturbation experiments we have validated the EGRIN-predicted role of two novel TFs in the regulation of phosphate-dependent repression of formate dehydorgenase – a key enzyme in the methanogenesis pathway.

2013-09-16 | GSE42164 | GEO

Time series of Methanococcus maripaludis MM901:del_MMP1100, a shift from a H2-excess and P-limiting condition to a H2-limited and P-excess condition in chemostats (growth rate held constant)

2013-09-16 | GSE42162 | GEO

Time series of Methanococcus maripaludis MM901:del_MMP0719, a shift from a H2-excess and P-limiting condition to a H2-limited and P-excess condition in chemostats (growth rate held constant)

2013-09-16 | GSE42159 | GEO

Time series of Methanococcus maripaludis MM901, a shift from a H2-excess and N-limiting condition to a H2-limited and N-excess condition in chemostats (growth rate held constant)

2013-09-16 | GSE42126 | GEO

Steady-state samples of Methanococcus maripaludis MM901 grown on formate-excess medium

2013-09-16 | GSE42115 | GEO

Time series of Methanococcus maripaludis MM901:del_fruA (MMP1382)-del_frcA (MMP0820), a shift from a H2-excess and P-limiting condition to a H2-limited and P-excess condition in chemostats (growth rate held constant)

2013-09-16 | GSE42165 | GEO

Time series of Methanococcus maripaludis MM901, a shift from a H2-limited condition to a H2-excess condition