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Single-cell RNA-seq of mouse thymus iNKT cells

ABSTRACT: Single-cell RNA-seq of mouse thymus iNKT cells

PROVIDER: PRJNA534242 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8944850_1.fastq.gz	Fastqsanger.gz
	SRR8944850_2.fastq.gz	Fastqsanger.gz
	SRR8944843_1.fastq.gz	Fastqsanger.gz
	SRR8944843_2.fastq.gz	Fastqsanger.gz
	SRR8944844_1.fastq.gz	Fastqsanger.gz

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Single-cell RNA-seq of mouse thymus iNKT cells

Project description:Invariant natural killer T cells (iNKT) cells are innate-like T cells, selected from thymic cortex-resident CD4+CD8+ double positive (DP) thymocytes. Despite major advances in the understanding of iNKT cells development, the heterogeneity of iNKT subsets and underlying molecular programs that guide iNKT cell-lineage remain unclear.

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Single-cell RNA-seq of mouse peripheral iNKT cells

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Project description:Invariant natural killer T cells (iNKT) cells are differently distributed in various immune organs. However, it remains unclear whether iNKT cells exhibit phenotypical and functional differences in different peripheral organs and how thymic iNKT migrate to peripheral organs. Hence, characterizing iNKT cells in peripheral organs is important.

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Project description:Transcriptional profiling of mouse iNKT cells comparing wild type and Bcl11b deficient cell. The mice were treated with 4 Î¼g of Î±-galactosylceramide. Goal was to determine the effects of transcription factor Bcl11b removal in iNKT cells. Intraperitoneal treatment with 4 Î¼g of Î±-Galactosylceramide. Two pair of wild type (BCL11b F/F VÎ±14 transgenic) and Knock out (BCL11b F/F PLZF-Cre VÎ±14 transgenic)) mice were treated. Lymphocytes from spleen and liver were enriched and stain with PBS-57 Loaded CD1d tetramer. Pure iNKT cells were collected through cell sorter.

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