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ABSTRACT: Endophytic bacteria in root of Russula forest

PROVIDER: PRJNA534403 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8948550_1.fastq.gz	Fastqsanger.gz
	SRR8948550_2.fastq.gz	Fastqsanger.gz
	SRR8948551_1.fastq.gz	Fastqsanger.gz
	SRR8948551_2.fastq.gz	Fastqsanger.gz
	SRR8948552_1.fastq.gz	Fastqsanger.gz

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Project description:H. seropedicae is a diazotrophic and endophytic bacterium that associates with economically important grasses promoting plant growth and increasing productivity. To identify genes related to bacterial ability to colonize and promote plant growth wheat seedlings growing hydroponically in Hoaglandâs medium were inoculated with H. seropedicae the bacteria and incubated for 3 days. mRNA from the bacteria present in the root surface and in the plant medium were purified, depleted from rRNA and used for RNA-seq profiling. RT-qPCR analyses were conducted to confirm regulation of selected genes. Comparison of RNA profile of bacteria attached to the root and planktonic revealed an extensive metabolic adaptation to the epiphytic life style.

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Project description:The objective of this work was to expand the knowledge about the possible mechanisms involved in the early stages of the interaction between the diazotrophic endophytic strain Kosakonia radicincitans UYSO10 and sugarcane plants. For that, a proteomic approach was conducted in the strain UYSO10 exposed or not to sugarcane exudates. Results showed that in the presence of root exudates the UYSO10 strain senses the environment and adapts its proteome to transport and metabolize different nutrients, and to interact with the host plant. These results deepen the knowledge of the potential mechanisms involved in the early stage of plant-bacteria endophyte interaction.

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